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末梢全血不同放置时间对白细胞及血小板检测的影响 被引量:18

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摘要 目的探讨末梢全血采集后不同放置时间对白细胞(WBC)和血小板(PLT)检测的影响。方法对40例患者采集末梢全血注入乙二胺四乙酸二钾(EDTA-K2)抗凝管抗凝,轻轻充分混匀后立即在血细胞分析仪检测,然后分别放置10 min和30 min后再次测定;对以上三个时间测定的白细胞数、中性粒白细胞百分率(NEUT%)、中间细胞面分率(MXD%)、淋巴细胞百分率(LYMPH%)、血小板数、血小板体积分布宽度(PDW)、血小板平均体积(MPV)和大血小板比值(P-LCR)结果进行分析比较。结果10 min的结果与30 min的结果比较:白细胞数降低,血小板数升高,NEUT%升高,LYMPH%降低,MPV降低、P-LCR降低,两组结果经配对t检验差异有显著性(P<0.01),其中血小扳数差异有极显著性(P<0.001),MXD%与PDW差异无显著性(P>0.05);30 min结果与10 min结果比较差异均无显著性(P>0.05);30 min的白细胞和血小板直方图均出现不同程度异常,10 min后直方图恢复正常。结论末梢全血在采集过程中由于标本或多或少有组织液混入及血小板周围环境的改变,促使血小板短时间内产生一个假性聚集现象,从而影响WBC和PLT结果的准确性,在EDTA-K2的逐渐作用下血小板解聚,结果恢复正常;末梢全血采集后放置10 min后再测可提高检测的准确性。
作者 苏钏
出处 《临床和实验医学杂志》 2007年第3期69-70,共2页 Journal of Clinical and Experimental Medicine
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