摘要
目的:研究大鼠肺纤维化形成过程中肺组织表达胰岛素样生长因子Ⅰ(IGF-Ⅰ)和转化生长因子β1(TGF-β1)的变化。方法:60只雄性Wistar大鼠,随机分为对照组20只及模型组40只,分别用生理盐水和博莱霉素(5 mg.kg-1)气管内灌注,于第7、14、21和28天处死,取肺组织,采用免疫组织化学方法检测肺组织中IGF-Ⅰ和TGF-β1的表达情况。结果:实验组经博莱霉素诱导4周后,取肺组织行HE染色,病理符合肺纤维化改变;苦味酸-酸性品红染色可见肺间质胶原纤维生成,证实肺纤维化模型形成。免疫组化显示,模型组肺组织中表达IGF-Ⅰ、TGF-β1阳性细胞分布于肺间质,阳性细胞百分率和表达强度与对照组比较差异有显著性(P<0.05);在第1、2和3周IGF-Ⅰ的表达逐渐增强,第4周出现下降,但第1周分别与第3周和第4周比较,差异仍有显著性(P<0.05);TGF-β1的表达高峰出现在第2周,第3周开始出现下降,但至第4周时,TGF-β1的表达水平仍高于第1周,第1周分别与第2、3周比较差异有显著性(P<0.05)。结论:IGF-Ⅰ和TGF-β1的高水平表达,促进了肺纤维化的形成;IGF-Ⅰ和TGF-β1共同参与了肺纤维化的形成,并可能存在协同作用。
Objective To study the expressions of IGF-Ⅰ and TGF-β1 in lung tissue of rats with pulmonary fibrosis. Methods Sixty male Wistar rats were randomly divided into two groups： control group （n= 20） and experimental group （n=40）, and poured respectively by saline and bleomycin solutions, and killed on the 7th, 14th, 21th, 28th day. Immunohistochemistry method was used to analyze the expressions of IGF-Ⅰ and TGF-β1 in lung tissue. Results After 4 weeks induction by bleomycin solutions, the lung tissue was obtained and stained by HE, the pathological changes were in accordance with pulmonary fibrosis. Pulmonary interstitial fibers were found by VG staining. So lung fibrosis models induced by bleomycin were set up successfully. The expressions of IGF-Ⅰ and TGF-β1 were increased during the progression of pulmonary fibrosis. Excellent differences of percentage of positive cells and expression level of IGF- Ⅰ and TGF-β1 were found between control group and experimental group （P〈 0. 05）. The expressions of IGF-Ⅰ were increased on the first, second, and third week, and decreased on the fourth week, however, there were excellent differences between the first and third week and between the first and fourth week （P〈0. 05）. The peak of TGF-β1 expression was on the second week, and decreased during the third week. But its expression on the fourth week was also higher than that on the first week. Excellent differences were found between the first week and the second or third week （P〈0.05）. Conclusion The high level expressions of IGF- Ⅰ and TGF-β1 have the vital significance for the formation of pulmonary fibrosis, both IGF- Ⅰ and TGF-β1 also take an effect on the pulmonary fibrosis formation, and may have synergetic effect.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2007年第2期231-233,共3页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题(30170887)
关键词
胰岛素样生长因子Ⅰ
转化生长因子Β
肺纤维化
免疫组织化学
insulin-like growth factor Ⅰ
transforming growth factor beta
pulmonary fibrosis
immunohistochemistry
