摘要
目的研究不同浓度的有机碘（DIT）对体外培养的人甲状腺细胞（TEC）增殖情况的影响,并且和无机碘（KI）进行比较,以探讨一种更安全有效的补碘制剂。方法取甲状腺腺瘤旁正常甲状腺组织,应用原代细胞培养技术进行细胞培养,以不同浓度（10-8~10-3mol/L）的DIT和KI刺激单层培养的甲状腺细胞,采用四甲基偶氮噻唑蓝（MTT）法测定各组甲状腺细胞的增殖情况。结果低浓度的KI对人甲状腺细胞生长没有影响,而中、高浓度的KI对细胞生长具有抑制作用,并且呈现剂量-时间依赖关系;与对照组相比,浓度越高,时间越长,抑制越明显。DIT组只有在高浓度才对甲状腺细胞出现抑制作用,并且DIT各剂量组与KI各剂量组在同一剂量水平进行两两比较发现,DIT对细胞的抑制作用明显轻于KI对细胞的抑制作用。结论高浓度的碘可以抑制甲状腺细胞的生长,DIT对细胞生长的抑制低于KI,即DIT对细胞的损伤较KI轻。
Objective To investigate the influence of DIT on the proliferation of human thyrocytes and compare DIT with KI. To study a good kind of supplement to USI that was safe and effective. Methods The human thyroid epithelium cells from para - adenoma normal tissues from thyroid adenoma patients were cultured in the absence or presence of 10^ -8- 10^-3mol/L DIT or KI. MTT method was employed to obtain the proliferation of cultured human thyrocytes. Results KI of middle and high dose can inhibit human thyrocytes to proliferate and exhibit dose - effect and time - effect relationships. DIT of only high dose can inhibit human thyrocytes proliferate. In the same dose of DIT and KI, the effect of DIT was less than KI on inhibition of cell proliferation. Conclusion High concentration of iodine can inhibit human thyrocytes to proliferate and the effect of DIT is less than KI. DIT has more security than KI.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2007年第4期427-428,共2页
Chinese Journal of Public Health
基金
山东省卫生厅科研基金资助(2001CAIAA18)
