摘要
实验以“绿杆芋”的茎尖为外植体,经脱毒快繁后将组培芋苗入土种植,作为组培苗第1代(T1),以T1的球茎作繁殖器官于第2年种植入土,作为组培苗第2代(T2).将T1,T2以及普通苗(CK)栽植在温室中,待芋苗抽生侧芽时,测定叶片中游离脯氨酸、超氧化物歧化酶、丙二醛、过氧化氢酶、叶绿素、可溶性糖、淀粉和蛋白质等生理指标.因子分析表明:8个指标被分成了3个主成分;T1,T2,CK在3个主成分上的综合得分分别是-0·448,0·517,-0·069,其大小顺序为T2,CK,T1.同时,测定了不同芋苗产量,T1,T2,CK单株平均产量分别是1·392kg,1·748kg,0·960kg,其大小顺序为T2,T1,CK,且T2显著高于T1和CK.综合评价考虑,组培2代T2要优于组培1代T1和普通苗CK,是大面积推广栽植的种源.
Surface-discontaminated tip buds of taro were used as explants in in vitro culture for rapid multiplication and the plants thus obtained （T1） were transplanted into soil. The corms of T1 were planted as multiplication organs the next year and the plants were designated as T2. T1 plants, T2 plants and normal plants （CK） were grown in a greenhouse and their leaves were used for the determination of proline content, SOD and CAT activities, MDA content and the contents of chlorophyll, soluble sugars, starch and proteins. Factor analysis showed that the 8 indexes determined were divided into 3 principal components and their total scores of T1, T2 and CK were -0. 448, 0. 517 and -0. 069, respectively, in the order of T2〉CK〉T1. Taro yield per plant was 1.392, 1.748 and 0.960 kg （T2〉T1〉CK）. Therefore, recommended that T2 plants should be used in large-scale cultivation.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第2期66-69,共4页
Journal of Southwest University(Natural Science Edition)
基金
重庆市农业局资助项目"蔬菜营养体快繁技术研究"
关键词
组织培养
芋头
因子分析
tissue culture
taro
factor analysis
