摘要
目的：建立积雪草中主要生物活性成分积雪草苷的HPLC—ELSD含量测定方法。方法：应用LabAlliance ELSD MODEL-200蒸发光散射检测器；色谱柱为Aichrom Reliasil C18柱（250mm×4．6mm，5um），以甲醇-水（55：45）为流动相，流速1．0mL／min，柱温为35℃，测定积雪草苷的含量。结果：积雪草苷在1．216-6．08ug之间呈良好的线性关系（r=0．9994），精密度、重现性RSD均小于2％，平均回收率为97．1％。结论：此方法简便快速、选择性好，适用于积雪草中积雪草苷的质量分析。
Objective： To develop a new method using HPLC - ELSD for determination content of Asiaficoside in the Herb of Asiatic Pennywort. Methods： LabAlliance ELSD MODEL -200 was used. The chromatorgraphic conditions was following： Aichrom Reliasil C18 Column （ 250 mm × 4. 6 mm , 5 um ） , the mobile phase was composed of methanol - water （ 55：45 ）, flow rate was at 1.0 mL/min, Column temperature was 35℃. Results： The calibration cures of Asiaticoside were within the range of 1. 216 - 6.08 ug （ r = 0. 9994） and the average recovery was 97.1% ; the precision and stability of samples （RSD） were below 2%. Conclusion： The method was shown to be accurate and convenient, and suitable for the quantitative analysis of Asiaticoside in the Herb of Asiatic Pennywort.
出处
《成都中医药大学学报》
2007年第1期54-55,57,共3页
Journal of Chengdu University of Traditional Chinese Medicine
