摘要
以枇杷Eriobotrya japonica叶为原料,乙醇为介质,研究超声波和回流2种提取方法对乌索酸的提取效果。用相同原料和相同介质,采用超声波和回流2种方式进行提取,再经滤过、定容、离心和微孔滤膜过滤后测定,用外标法计算质量分数。建立了枇杷叶中乌索酸质量分数的高效液相色谱定量方法;超声波提取的效率为热回流法的2.63倍。枇杷叶用超声波提取时,对乌索酸的提取效率远优于热回流法。超声波具有独特的机械、空化和热效应,能使介质分子的运动速度加大,穿透力增强,从而使有效成分充分溶出。
The conventional methods to extract ursolic acid is not satisfied. Ethyl alcohol with ultrasonic waves and backflow were used to extract ursolic acid from Eriobotrya japonica （loquat） leaves to determine the methods＇ effects. A single raw material source （E. japonica dry leaves） and medium （ethyl alcohol） were filtered, centrifuged, and filtered again with a micropore filter diaphragm; then, two different methods, ultrasonic waves and backflow, were used to extract ursolic acid which was quantified using high performance liquid chromatography （HPLC） with the external standard method. The extracting efficiency of ultrasonic waves was 2.63 times greater than hot backflow. The ultrasonic waves had unique mechanical, cavitating, and thermal reactions, which increased the speed and penetration of the medium molecules, thereby enabling greater extractron of the ursolic acid. [ Ch, 3 fig. 2 tab. 7 ref. ]
出处
《浙江林学院学报》
CSCD
北大核心
2007年第2期239-241,共3页
Journal of Zhejiang Forestry College
基金
国家高技术研究发展计划(863计划)项目(2002AA2Z3217)
关键词
植物学
枇杷叶
超声波
回流技术
乌索酸
提取效果
botany
Eriobotrya japonica （loquat） leaf
ultrasonic technology
backflow technology
ursolic acid
extract effect
