摘要
目的探讨山楂叶总黄酮(TFHL)对大鼠肾缺血/再灌注(I/R)的作用。方法采用在体夹闭双侧肾动脉60min后恢复灌注的方法复制大鼠肾I/R损伤的模型,夹闭动脉前30min静脉注射TFHL30或60mg/kg。检测再灌注1、24h后血清BUN、血Scr及TNF-α和IL-1水平及再灌注24h后肾组织丙二醛(MDA)和超氧化物歧化酶(SOD)水平。结果TFHL能显著降低I/R导致的血清BUN和Scr升高,增加尿量,减少TNF-α和IL-1产生,提高肾组织SOD活性,减少MDA生成。结论TFHL能明显减轻肾I/R损伤,有效改善肾功能,其机制与TFHL减少脂质过氧化反应,减少细胞因子TNF-α和IL-1水平有关。
Objective To study the protective effect of total flavones of hawthorn leaf （TFHL） on renal ischemia/reperfusion （I/R） in rats. Methods A model of renal I/R was made by clamping double renal pedical for 60min and reperfusion for 24 h. TFHL （30 or 60 mg/kg） was injected before clamping renal ischemia. The level of serum urea nitrogen（BUN） , serum creatinine（ Scr）, tumor necrosis factor -α （ TNF -α）, interleukin - 1 （IL - 1 ） in serum were detected after reperfusion for 1 and 24 h, respectively, and the level of malonaldehyde （MDA） and erythrocuprein （SOD） in renal tissues were measured after reperfusion for 24 h. Results TFHL significantly decreased the level of BUN and Scr, decreased the concentration of TNF-α and IL - 1 in serum. Pretreatment with TFHL also enhanced the activity of SOD reduced the concentration of MDA in renal after I/R. Conclusions TFHL has significantly protective effect on renal I/R and markedly improve the renal function. The mechanisms are associated with decreasing the lipid peroxidation reaction and reducing the level of TNF -α and IL - 1.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2007年第5期359-360,共2页
Journal of Applied Clinical Pediatrics
关键词
山楂叶总黄酮
缺血
再灌注损伤
大鼠
total flavones of hawthorn leaf
ischemia
reperfusion injury
rat
