摘要
目的探讨胰岛素样生长因子-1(IGF-1)及其相关蛋白在病毒性心肌炎病程中的作用。方法取Balb/C小鼠60只,随机分为病毒实验组40只,腹腔感染CVB3病毒,分别于实验d3、7、15、30采血、取心肌组织;空白对照组(未感染CVB3病毒小鼠)20只。用酶联免疫吸附测定法(ELISA)检测其血浆IGF-1的表达,用免疫组织化学法和逆转录酶多聚链反应(RT-PCR)技术检测其心肌组织IGF-1及相关蛋白的表达。结果病毒性心肌炎小鼠血浆IGF-1及相关蛋白表达均较空白对照组高,且在感染病毒后表达渐增高,15 d达最高峰,30 d表达降低,但仍维持一较高水平,与对照组比较仍有显著差异(F=19.53 P<0.05),其在心肌组织中的表达与血浆中的表达变化规律一致。结论IGF-1及其相关蛋白可能参与小鼠CVB3病毒性心肌炎的病理生理过程。
Objective To investigate the changes of insulin - like growth factor - 1 （ IGF - 1 ） and its associated protein in Balb/C mice with viral myocarditis. Methods Sixty Balb/C mice were randomly divided into 2 groups：infected group（ n = 40） and control group （ n = 20 ）. Mice in infected group were inoculated intraperitoneally with coxsackievirus B3 （ CVB3 ） while control mice with 0.1 mL of Eagle＇s minimal essential medium（EMEM）. Blood and myocardial musculature of all mice were collected on the day of 3,7,15 and 30 after inoculation. The expression of IGF - 1 and its associated protein in plasma or myocardium were detected respectively by enzyme - labeled immunosorbent assay（ ELISA）, immunohistochemistry and reverse transcription polymerase chain reaction（ RT - PCR）. Results The expression of IGF - 1 and its associated protein of mice with viral myocarditis was higher than those in control group and gradual ascent after mice being infected with virus. The expression reached summit on d15, then decreased on d30, but still sustain in a high level. The difference was significant（F = 19.53 P 〈 0.05）. The expression change axiom of IGF - 1 and its associated protein in myocardium was in conformity with that in plasma blood. Conclusion IGF - 1 and its associated protein may participate in pathophysiological processes of viral myocarditis.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2007年第6期443-444,457,共3页
Journal of Applied Clinical Pediatrics
基金
国家自然科学基金项目资助(30271665)
