摘要
目的研究碘过量对Wistar成年大鼠海马星形胶质细胞的影响。方法断乳一个月Wistar大鼠,雌雄各半,随机分为4组适碘组(NI),10倍碘组(10HI),50倍碘组(50HI)和100倍碘组(100HI)。给予不同浓度碘酸钾水喂养6个月后,处死,取大脑,应用免疫组织化学技术观察海马CA3区星形胶质细胞,并对胶质原纤维酸性蛋白GFAP反应阳性细胞进行形态计量学分析。结果各碘过量组海马GFAP阳性细胞的Vv,NA和细胞质的平均灰度值与NI组比较均无明显差异(P>0.05)。结论碘过量不会造成Wistar成年大鼠海马神经元的明显损伤,其星形胶质细胞Vv,NA和细胞质的平均灰度值无明显改变。
Objective To study the effects of iodine excess on astrocytes of hippocampus in Wistar rat. Methods Wistar rats at the age of two month, half male and half female, were divided into four groups randomly： normal iodine group （ NI group）, 10, 50 and 100 folds of normal iodine intake group （10HI, 50HI and 100HI groups）. After fed with different concentration iodine diet for 24 weeks, the morphology change of the hippocampal astrocytes in Wistar rats were observed. The expression of GFAP were examined by immunohistochemistry, and volume density （ Vv）, numerical density on area （NA） and the mean of grey scale were measured. Results Compared with NI group, the Vv, NA and the mean of grey scale of astrocytes in 10HI, 50HI and 100HI groups were of no statistical difference（ P 〉 0. 05）. Conclusions Iodine excess can not injure the hippocampal neurons in rats.
出处
《中国地方病防治》
北大核心
2007年第2期81-83,共3页
Chinese Journal of Control of Endemic Diseases
基金
国家自然科学基金重点资助项目(30230330)
关键词
碘过量
GFAP
海马
Iodine excess
GFAP
Hippocampus
