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丹参酮Ⅱ-A磺酸钠对纤维化人肾间质成纤维细胞体外增殖及cyclin E蛋白表达的影响 被引量:35

Effect of sodium tanshinone Ⅱ-A sulfonate on proliferation and cyclin E expression in human renal interstitial fibroblasts in vitro
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摘要 目的研究丹参酮Ⅱ-A磺酸钠(sodium tanshinoneⅡ-A sulfonate,DS-201)对人肾间质纤维化来源的成纤维细胞(human renal interstitial fibroblasts,hRIFs)体外增殖及细胞周期素E(cyclin E)基因表达的影响,探讨该药治疗肾间质纤维化的作用机制。方法体外培养并鉴定hRIFs;用四甲基偶氮唑(MTT)法检测对照组与不同DS-201浓度组hRIFs的增殖活性;用免疫细胞化学S-P法和图像分析技术检测对照组与不同DS-201浓度组hRIFs cyclin E基因的表达。结果随药物浓度的升高和作用时间的延长,抑制率逐渐升高,抑制作用逐渐增强,呈剂量依赖性和时间依赖性。用药组阳性细胞的平均光密度值在第3、5、7、9天显著低于对照组(P<0.05,P<0.01),第1天用药各组与对照组之间无统计学差异(P>0.05)。结论①DS-201对hRIFs体外增殖有显著抑制作用,可能是其治疗肾间质纤维化的机制之一。②DS-201抑制hRIFs体外增殖可能是通过抑制细胞中cyclin E基因的表达,阻滞细胞通过G1/S关卡,延长细胞周期实现的。 Objective To study the effect of sodium tanshinone Ⅱ-A sulfonate ( DS-201 ) on the proliferation of human renal interstitial fibroblasts (hRIFs) and the expression of cyclin E. Methods After hRIFs were cultured and identified, MTT was used to detect the cells at passage 3-4 in present or absent of DS-201at different concentrations. The expression of cyclin E was analyzed by immunocytochemistry and the image-analytical technique. Results With the increase of the drug concentration and prolongation of action time, the inhibitory ratio was increased in a dose- and time-dependent manner. The expression of cyclin E was stronger in drug groups than in control group on the 3rd, 5th, 7th and 9th day (P 〈 0.05,P 〈 0.01 ). Conclusion DS-201 significantly inhibits the hRIFs in vitro,which may be one of its mechanisms to suppress renal interstitial fibrosis. The mechanism of this inhibition may be due to that sodium tanshinone Ⅱ-A sulfonate inhibits the expression of cyclin E, thus blocks the G1/S point and extents the cell cycle.
出处 《第三军医大学学报》 CAS CSCD 北大核心 2007年第7期585-587,共3页 Journal of Third Military Medical University
基金 四川省中医药管理局科研基金(200022)~~
关键词 丹参酮Ⅱ-A磺酸钠 肾脏 纤维化 成纤维细胞 细胞周期素E sodium tanshinone Ⅱ-A sulfonate renal interstitium fibrosis fibroblasts cyclin E
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  • 1于德民,吴锐,尹潍,袁咏.实验性链脲佐菌素糖尿病动物模型的研究[J].中国糖尿病杂志,1995,3(2):105-109. 被引量:425
  • 2Matsuoka LY, Uitto J, Wortsman J, et al. Ultmsmn,A characteristics of keloid fibroblasts [J]. Dermatopathol. 1988; 10(6) : 505.
  • 3Rodeman HP, Muller GA, Knecht A, et al. Fibroblasts of rabbit kidney in culture: Chamcterozation and identification of cell-specific markers [J]. Am J Physiol. 1991; 261 (2):283.
  • 4Luo S, Benathan M, raffoul W, et al. Abnormal balance between proliferation and apoptotic cell death in fibroblasts derived from keloid lesions.Plast Reconstr Surg, 2001, 107(1):87
  • 5Kasahara Y, Tuder RM, Cool CD, et al. Endothelial cell death and decreased e xpression of vascular endothelial growth factor and vascular endothelial growth factor receptor 2 in emphysema. Am J Respir Crit Care Med, 2001,163: 737
  • 6Akasaka Y, Fujita K, Ishikawa Y, et al. Detection of apoptosis in keloids and a comparative study on apoptosis between keloids, hypertrophic scars,normal healed flat scars, and dermatofibroma. Wound Repair Regen,2001,9(6) :501
  • 7陕声国 张端莲 候祚琼.瘢痕疙瘩成纤维细胞的增殖和凋亡状态[J].中华皮肤科杂志,2000,33(4):280-280.
  • 8王伟铭,姚建,曹红娣,楼鼎秀.人肾间质细胞培养的研究[J].上海第二医科大学学报,1998,18(6):455-457. 被引量:15
  • 9程丽静,谌贻璞,高进.人肾间质成纤维细胞的培养与鉴定[J].北京医科大学学报,2000,32(1):79-81. 被引量:28
  • 10陈明,曾瑜,黄颂敏,刘先蓉.糖尿病大鼠早期肾组织中TGFβ1的动态表达及意义[J].泸州医学院学报,1999,22(4):285-287. 被引量:3

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