摘要
目的探讨螺旋环螺旋基因家族(helix-loop-helix,HLH)成员DNA结合抑制因子2(inhibitor of DNA bind-ing2,Id2)在大鼠脊髓中的表达。方法Zambonis液灌注固定大鼠脊髓组织,切片进行免疫组织化学(ABC法)染色。结果Id2免疫阳性细胞在脊髓中广泛分布,在Ⅰ～Ⅲ板层及Ⅸ板层Id2主要在细胞核表达,细胞质表达少。Ⅳ～Ⅷ板层细胞质及细胞核均有Id2表达。从Ⅳ到Ⅷ板层,Id2细胞质阳性细胞体积逐渐增大,染色较深。结论Id2广泛分布在大鼠脊髓灰质各个板层,不同板层中Id2的细胞定位不同,可能分布于不同类型的细胞中。
Objective To investigate the expression of the helix-loop-helix （HLH） transcription factor Id2 （inhibitor of DNA binding-2） in spinal cord of adult rats. Methods Immunohistochemistry was performed on the sections of spinal cord of rats perfused with Zambonis. Results The positive Id2-immunoreactive cells distributed in the spinal cord extensively. In lamina Ⅰ -Ⅱ, the expression of Id2 was predominantly in nucleus and few in cytoplasm, the same as lamina Ⅸ. In lamina Ⅳ-Ⅷ, positive Id2 immunoreactivity was present in both cytoplasm and nucleus. The volume of positive cells was larger and their staining got deeper from lamina Ⅳ to lamina Ⅷ gradually. Conclusion Id2 protein is widely distributed in grey matter of spinal cord of rats. The cellular location of Id2 different in laminas might be associated with cell type.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第7期592-594,共3页
Journal of Third Military Medical University
关键词
ID2
大鼠
脊髓
免疫组织化学
Id2
rat
spinal cord
immunohistochemistry DNA
