摘要
应用多重引物ＰＣＲ技术对４７例复发性呼吸道乳头状瘤病（ＲＲＰ）和９例声带息肉石蜡包埋组织中ＨＰＶ６／１１、１６、１８ＤＮＡ进行了检测。结果发现：①ＲＲＰ组织中ＨＰＶ６／１１ＤＮＡ的阳性率为６８．１％（３２／４７），其中喉乳头状瘤、口咽乳头状瘤、鼻腔和鼻前庭乳头状瘤组织中ＨＰＶ６／１１ＤＮＡ的阳性率分别为７０．４％（１９／２７）、６６．７％（４／６）和６４．３％（９／１４）；②所有ＲＲＰ标本中均未发现特异性的ＨＰＶ１６和ＨＰＶ１８ＤＮＡ；③９例声带息肉标本中四种型号的ＨＰＶＤＮＡ全部阴性。实验结果表明，ＲＲＰ的发病与ＨＰＶ６／１１感染密切相关。多重引物ＰＣＲ检测ＨＰＶＤＮＡ具有敏感、特异、快速、简便等优点，适合于临床广泛开展和进行流行病学调查。
Abstract Formalin-fixed,paraffin-embeded tissues from 36 patients with recurrent respiratory papillomatosis(27 laryngeal papillomas,6 oropharyngeal papillomas and 3 nasal vestibular papillomas)and 9 patients with cordal polyps were evaluated for the presence of HPV6/11、16、18DNA using polymerase chain reaction(PCR)with multiple primers。HPV6/11 DNA was found in 19 of 27 laryngeal papillomas,4 of 6 oropharyngeal papillomas and 2 of 3 nasal vestibular papillomas.HPV16,18 DNA was negative in all papilloma specimens.No HPV DNA was found in cordal polyps.These results showed that HPV was highly implicated in the pathogenesis of recurrent respiratory papillomatosis.The multiple primer PCR techinique is a useful tool for the detection of HPV
出处
《耳鼻咽喉（头颈外科）》
1996年第3期141-144,共4页
Chinese Arch Otolaryngology-Head Neck Surg
