摘要
目的：建立夏桑菊颗粒中蒙花苷的含量测定方法。方法：采用HPLC法，色谱柱：C18（Hypersil ODS 250mm×4．6mm，5μm，大连依利特公司），柱温：25℃。流动相：甲醇-水-冰乙酸（24：25：1），检测波长：334Bin，流速：1．0ml／min。结果：回归方程：Y=1047294．969X＋6064．236，γ=0．9995。结果表明蒙花苷在0．0408～0．3672μg范围内具有良好的线性关系。平均回收率为99．76％，RSD％=0．72％。结论：该方法准确可靠，重现性好，可用于夏桑菊的质量控制。
Objective：To establish the method for determination of the content of linarin in Xiasangju granules. Method： HPLC method was used. The chromato bar was C18 column （ Hypersil ODS 250 mm ×4.6 mm,5 μm） with mobile phase methanol - water - glacial acetic acid（24 ： 25 ： 1 ） at the temperature of 25 ℃. The detection wavelength was 334 nm, the flow rate was 1.0 ml/min. Result ： regression equation： Y = 1047294. 969X ＋ 6064. 236, r = 0.9995. the result showed that linarin had a linear relationship at the range of 0.0408- 0.3672 μg. The average recovery was 99.76% ,RSD% = 0.72%. Conclusion： The method is accurate, reliable with well reproducible, can be used to control the quality of Xiasangju granules.
出处
《中医药导报》
2007年第4期79-80,97,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
关键词
HPLC
蒙花苷
含量测定
HPLC
linarin
Determination of the content
