摘要
以金叶日本冬青新梢茎段为外植体,探讨不同部位新梢、不同激素组合、糖源以及培养基pH值对其愈伤组织诱导及分化的影响。结果表明,采用相同处理,取中上部新梢茎段作外植体较好,其消毒成活率、愈伤萌动率、分化成苗率均较高;MS+KT 1.0 mg/L+NAA 0.2mg/L培养基对愈伤组织诱导分化最佳;蔗糖浓度为30 g/L时效果最好,愈伤诱导率为100%;培养基pH值以6.0较适宜。
In this experiment the effect of homaone combination, sugar source and pH on callus induction and differentiation was investigated. The results indicated that new stem-tip at upper part was optimum explant,with high survival rate after sterilization and the sprouting rate of its callus were relatively high. MS medium supplemented with KT 1.0 mg/L, and NAA 0.2 mg/L was the best one for callus induction and differentiation. When the density of sugar was 30 g/L, the rate of callus induction was 100% .In addition,the optimum medium pH was 6.0.
出处
《安徽农业科学》
CAS
北大核心
2007年第9期2569-2570,共2页
Journal of Anhui Agricultural Sciences
