期刊文献+

乙草胺对小麦生理机能的影响与生物标记物识别 被引量:11

Effects of Herbicide Acetochlor on Physiological Mechanisms in Wheat and Biomarkers Identification
下载PDF
导出
摘要 对乙草胺胁迫下小麦幼苗叶片的超氧化物歧化酶(SOD)、过氧化物酶(POD)活性、可溶性蛋白含量、丙二醛(MDA)以及叶片中叶绿素含量的变化进行了研究.结果表明,乙草胺处理小麦幼苗1d后,叶片MDA含量显著增加,说明了乙草胺处理诱导有毒活性氧(AOS)生成,但随着处理时间的延长,不同浓度处理与对照的差异逐渐减小.对小麦叶片中POD活性变化的研究表明,在乙草胺胁迫的开始阶段,植物有能力抵抗低浓度乙草胺污染所产生的氧化胁迫,但是,随着暴露时间的延长和污染物浓度的增加,这种抵抗能力将会消失.对小麦SOD活性的研究表明,小麦叶片中POD酶活的上升可能与其它途径而不是SOD诱导产生的H2O2有关.小麦叶片中MDA含量和POD活性不能作为土壤乙草胺污染的生物标记物.小麦叶片中SOD酶活性有着作为乙草胺污染土壤生物标记物的潜力,但还需要进一步研究.小麦叶片叶绿素含量和可溶性蛋白含量可以作为乙草胺污染胁迫的生物标记物,并且小麦幼苗叶片可溶性蛋白含量与土壤乙草胺浓度之间具有较好的剂量-效应关系. Changes of the activity of antioxidant enzymes including peroxidases (POD) and superoxide dismutases (SOD) and the contents of chlorophyll (CHL), malondialdehyde (MDA), and soluble proteins (SP) in wheat ( Triticum aestivum L. ) under the stress of acetochlor in soil were examined. The increased MDA level detected in the leaves of wheat after 1 day exposure of acetochlor indicated the presence of poisoning AOS. However, the difference of MDA levels in leaves of different concentrations of acetochlor decreased with the prolonged exposure. The data of POD could suggest that the plant had the capacity to counteract the oxidative stress caused by lower concentration of acetochlor, but the capacity would be lost with exposure time. It is indicated that the increase of POD activity in the leaves may be caused by H2O2 produced from sources other than SOD. The activity of POD and contents of MDA could not he considered as biomarkers of stress by acetochlor in soil. While the activity of SOD could be considered as biomarkers of stress by acetochlor in soil need farther research. But the contents of CHL and SP could be considered as biomarkers of stress by acetochlor in soil and there are dose-response relationships between the SP content in leaves and the concentration of acetochlor in soil.
出处 《环境科学》 EI CAS CSCD 北大核心 2007年第4期866-871,共6页 Environmental Science
基金 国家重点基础研究发展规划(973)项目(2004CB418503) 国家杰出青年科学基金项目(20225722) 国家自然科学基金重点项目(20337010)
关键词 生态毒理 抗氧化酶 乙草胺 生物标记物 小麦(Triticum AESTIVUM L.) ecotoxicological effect antioxidant enzyme acetochlor biomarker wheat ( Triticum aestivum L. )
  • 相关文献

参考文献25

  • 1Lolpin D W,Nations B K,Goolsby D A.Acetochlor in the hydrologic system in the Midwestern United States[J].Environ.Sci.Technol.,1996,30:1459 ~ 1464.
  • 2朱九生,乔雄梧,王静,秦曙.乙草胺在土壤环境中的降解及其影响因子的研究[J].农业环境科学学报,2004,23(5):1025-1029. 被引量:53
  • 3Xiao H,Zhou Q X,Liang J D.Single and joint effects of acetochlor and urea on earthworm Esisenia foelide populations in phaiozem[J].Environ.Geochem.Health,2004,26:277~ 283.
  • 4郑和辉,叶常明.乙草胺和丁草胺在土壤中的移动性[J].环境科学,2001,22(5):117-121. 被引量:50
  • 5Boyd R A.Herbicides and herbicide degradates in shallow groundwater and the Cedar River near a municipal well field,Cedar Rapids,Iowa[J].Sci.Total.Environ.,2000,248:241~ 253.
  • 6Dagnac T,Jeannot R,Mouvet C,et al.Determination of oxanilic and sulfonic acid metabolites of acetochlor in soils by liquid chromatography-electrospray ionization mass spectrometry[J].J.Chromatogr.A,2002,957:69 ~ 77.
  • 7Hill A B,Jefferies P R,Quistad G B,et al.Dialkylquinoneimine metabolites of chloroacetanilide herbicides induce sister chromatid exchanges in cultured human lymphocytes[J].Mutat.Res.Genet.Toxicol.Environ.Mutagen.,1997,395:159~171.
  • 8Ashby J,Tinwell H,Lefevre P A,et al.Evaluation of the mutagenicity of acetochlor to male rat germ cells[J].Mutat.Res.Genet.Toxicol.Environ.Mutagen.,1997,393:263~ 281.
  • 9Crump D,Werry K,Veldhoen N,et al.Exposure to the herbicide acetochlor alters thyroid hormone-dependent gene expression and metamorphosis in Xenopus laevis[J].Environ.Health.Perspect.,2002,110:1199~ 120.
  • 10周启星,孙福红,郭观林,孙铁珩.乙草胺对东北黑土铅形态及生物有效性的影响[J].应用生态学报,2004,15(10):1883-1886. 被引量:17

二级参考文献67

  • 1龚光明.乙草胺防除水稻移栽田杂草[J].农药,1994,33(1):42-42. 被引量:5
  • 2杨柏云,熊冬金,黄亚萍,朱友林,江陆斌,黄洪辉.适度低温对棉苗细胞保护酶系统的影响[J].南昌大学学报(理科版),1996,20(4):320-323. 被引量:4
  • 3马成仓,洪法水,李清芳.Hg浸种对玉米种子萌发过程中几种酶活性的影响[J].应用生态学报,1997,8(1):110-112. 被引量:25
  • 4张福锁.环境胁迫与植物营养[M].北京:北京农业大学出版社,1993.71-100.
  • 5邓碧玉 袁勤生 等.改良的联苯三酚自氧化测定超氧化物歧化酶活性的方法[J].生物化学与生物物理进展,1991,18(2):163-165.
  • 6林玉环 王夔.汞的生物化学作用.生命科学中的微量元素(第2版)[M].北京:中国计量出版社,1996.903.
  • 7中国土壤学会农业化学专业委员会.农业土壤化学常规分析方法[M].北京:中国科学出版社,1983.72-74.
  • 8陈学敏 王夔.自然界的铅.生命科学中的微量元素(第2版)[M].北京:中国计量出版社,1996.934-936.
  • 9[2]U. S. Environmental Protection Agency. Prevention, pesticides and toxic substances, Questions and answers. Conditional registration of acetochlor; U. S. EPA, Washington D C. 1994: 18.
  • 10[3]Jablonkai I, hulesch A, Barta I. Factors affecting the phototoxicity of the herbicideacetochlor to monocotyledonous and dicotyledonous weeds [ J ].Pesticides Sciences, 1997, 49 (4): 395 - 397.

共引文献445

同被引文献211

引证文献11

二级引证文献90

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部