摘要
本项研究对124例喉不同病变的新鲜组织标本,采用共同引物和多重引物PCR的方法,进行HPVDNA检测。用共同引物PCR检测HPV_(6,11,16,18,31,33,35,42,58)九个型别的感染,阳性病例再用多重引物PCR进一步分型。结果,①喉癌组:HPV感染的总阳性率为49.1%,其中HPV_(18)型阳性率15.8%,HPV_(16)型为12.3%,HPV_(16)和_(18)双重型感染为5.3%,HPV_(6/11)和_(18)型混合感染为3.5%,其它型为12.3%。②颈转移淋巴结组:总阳性率为21.4%,其中HPV_(16)、HPV_(18)及HPV_(16)和_(18)双重型感染各为7.1%。③癌前病变组:HPV感染阳性率为11.1%,为HPV_(6/11)和_(18)型混合感染。④声带息肉组:阳性率7.1%,为HPV_(6/11)型感染。⑤癌旁及癌周正常喉组织:均为HPVDNA阴性。本文对HPV在喉癌中致病作用进行了讨论。
Abstract HPV-DNA detection byapplying consensus primers and multiple primers of modified polymerase chain reaction( PCR)to 124 cases of fresh tissue samples with different lesions of larynx was presented.The resuIts showed that:(Ⅰ)The group of laryngeal carcinoma:The total rate of positive cases with HPV infection was 49.1%(28/57),where the rate for HPV_(18) was 15.8%(9/57),HPV_(16) was 12.3%(7/57),for HPV_(16) and HPV_(18) dual infection was 5.3%(3/57),for HPV_(6/11) and HPV_(18) mixed infection was 3.5%(2/57),and for other types was 12.3%(7/57).(Ⅱ)Neck metastatic lymphnode group:Out of 14 cases of neck metastatic lymphnode,3 cases with positive HPV,resulting in a total rate of positive cases was mounted to 21.4%(3/14),among which,there was one case of HPV_(16) and HPV_(18) infec-tion respectively,and one case of HPV_(16) and HPV_(18) dual infection,resulting in a rate at 7.1%for the respective cases immediately above(Ⅲ)Precarcinous lesions group:The rate for positive case with HPV infection was 11.1%(1/9)which were mixed infection of HPV_(6/11) and HPV_(18).(Ⅳ)Vocal cord polypus group:The rate of positive reaction was 7.1%(1/14),which were HPV_(6/11) infection.(Ⅴ)Nomal laryngeal tissue group:15 cases of normallaryngeal tissue adjacent to the carcinoma and15 cases of normal laryngeal tissue opposite tothe carcinoma were HPV-DNA negative. Thecarcinogenic action of HPV in laryngeal carcinoma was disscussed.
出处
《耳鼻咽喉(头颈外科)》
1996年第5期285-288,共4页
Chinese Arch Otolaryngology-Head Neck Surg