摘要
目的研究五味子中五味子醇甲的最佳提取、纯化工艺。方法以五味子醇甲及其提取物为指标,采用L9(34)正交试验优选合理的水提工艺,采用平行试验优选醇沉工艺参数。结果优选出的五味子醇甲提取、纯化工艺为用10倍量的水,提取3次,每次1.5h,滤液浓缩到1.4g生药·mL-1后醇沉至80%,醇液过滤,pH值调至7。结论本方法可为提取五味子的生产工艺提供参考。
OBJECTIVE： To optimize the process for extraction and purification of Schisandrin in Fructus Schisandra Chinensis. METHODS： The orthogonal test L9（34） was adopted to optimize the water extraction process using Schisandrin and its extracts as indicators. The parallel test was used to optimize the parameters of the alcohol precipitation technique. RESULTS： The optimized process for extraction and purification of Schisandrin was as follows： extracting three times using 10- fold water, 1.5h each time, with filter liquids concentrated to 1.4g of crude drug mL ^-1 then precipitated to 80% ; filtering alcohol liquids, and adjusting pH value to 7.CONCLUSIONS： This method can be used as a reference for the extraction of Fructus Schisandra Chinensis.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第12期896-898,共3页
China Pharmacy
关键词
正交试验
五味子醇甲
提取工艺
纯化
Orthogonal test
Schisandrin
Extraction process
Purification
