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Changes in Thermostability of Photosystem Ⅱ and Leaf Lipid Composition of Rice Mutant with Deficiency of Light-harvesting Chlorophyll a/b Protein Complexes 被引量:2

Changes in Thermostability of Photosystem Ⅱ and Leaf Lipid Composition of Rice Mutant with Deficiency of Light-harvesting Chlorophyll a/b Protein Complexes
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摘要 We studied the difference in thermostability of photosystem Ⅱ (PSII) and leaf lipid composition between a T-DNA insertion mutant rice (Oryza sativa L.) VG28 and its wild type Zhonghuau. Native green gel and SDS-PAGE electrophoreses revealed that the mutant VG28 lacked all light-harvesting chlorophyll a/b protein complexes. Both the mutant and wild type were sensitive to high temperatures, and the maximal efficiency of PSII photochemistry (FJ Fm) and oxygen-evolving activity of PSII in leaves significantly decreased with increasing temperature. However, the PSII activity of the mutant was markedly more sensitive to high temperatures than that of the wild type. Lipid composition analysis showed that the mutant had less phosphatidylglycerol and sulfoquinovosyl diacylglycerol compared with the wild type. Fatty acid analysis revealed that the mutant had an obvious decrease in the content of 16:1t and a marked increase in the content of 18:3 compared with the wild type. The effects of lipid composition and unsaturation of membrane lipids on the thermostability of PSII are discussed. We studied the difference in thermostability of photosystem Ⅱ (PSII) and leaf lipid composition between a T-DNA insertion mutant rice (Oryza sativa L.) VG28 and its wild type Zhonghuau. Native green gel and SDS-PAGE electrophoreses revealed that the mutant VG28 lacked all light-harvesting chlorophyll a/b protein complexes. Both the mutant and wild type were sensitive to high temperatures, and the maximal efficiency of PSII photochemistry (FJ Fm) and oxygen-evolving activity of PSII in leaves significantly decreased with increasing temperature. However, the PSII activity of the mutant was markedly more sensitive to high temperatures than that of the wild type. Lipid composition analysis showed that the mutant had less phosphatidylglycerol and sulfoquinovosyl diacylglycerol compared with the wild type. Fatty acid analysis revealed that the mutant had an obvious decrease in the content of 16:1t and a marked increase in the content of 18:3 compared with the wild type. The effects of lipid composition and unsaturation of membrane lipids on the thermostability of PSII are discussed.
出处 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2007年第4期515-522,共8页 植物学报(英文版)
关键词 high temperature lipid composition light-harvesting a/b protein complexes photosystem II rice. high temperature lipid composition light-harvesting a/b protein complexes photosystem II rice.
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参考文献1

  • 1LIN Zhifang1,PENG Changlian1,Xu Xinlan1,LIN Guizhu 1 & ZHANG Jingliu2 1.South China Institute of Botany,Chinese Academy of Sciences,Guangdong Key Laboratory of Digital Botanical Garden,Guangzhou 510650,China,2.National Key Laboratory of Plant Molecular Genetics,Institute of Plant Physiology & Ecology,Shanghai Institutes of Biological Sci- ences,Chinese Academy of Sciences,Shanghai 200032,China.Thermostability of photosynthesis in two new chlorophyll b-less rice mutants[J].Science China(Life Sciences),2005,48(2):139-147. 被引量:4

二级参考文献10

  • 1Zhi-Fang Lin,Chang-Lian Peng,Gui-Zhu Lin,Jing-Liu Zhang.Alteration of Components of Chlorophyll-Protein Complexes and Distribution of Excitation Energy Between the Two Photosystems in Two New Rice Chlorophyll b-less mutants[J].Photosynthetica.2003(4)
  • 2Zhi-Fang Lin,Chang-Lian Peng,Gui-Zhu Lin,Zhi-Ying Ou,Cheng-Wei Yang,Jing-Liu Zhang.Photosynthetic Characteristics of Two New Chlorophyll b-Less Rice Mutants[J].Photosynthetica.2003(1)
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