摘要
背景:消化性溃疡是临床常见病且易复发,溃疡愈合质量与其复发有密切关系。目的:研究壳聚糖对大鼠胃溃疡愈合质量的影响及其可能机制。方法:大鼠随机分为空白对照组、两组药物对照组(硫糖铝和雷尼替丁)和三组实验组(壳聚糖、壳聚糖+硫糖铝或雷尼替丁)。给药14天后测量溃疡面积并行HE染色、AB-PAS、第Ⅷ因子和SP法免疫组化染色,测定一系列溃疡愈合质量指标以及表皮生长因子(EGF)、碱性成纤维细胞生长因子(bFGF)和诱生型一氧化氮合酶(iNOS)的表达。结果:实验组溃疡面积较空白对照组和药物对照组显著缩小(P<0.01);再生黏膜厚度较空白对照组增加,囊状扩张腺体数量减少,腺体层数增加,PAS阳性面积增加,肉芽组织中新生微血管数量增多(P<0.01)。壳聚糖+硫糖铝或壳聚糖+雷尼替丁组的囊状扩张腺体数量显著小于雷尼替丁组(P<0.05),腺体层数显著大于三者单用(P<0.05),PAS阳性面积和新生微血管数量显著大于雷尼替丁组(P<0.05)。各实验组EGF和bFGF的表达均显著高于空白对照组和雷尼替丁组(P<0.05),但iNOS在各组间的表达无显著差异。结论:壳聚糖可提高大鼠胃溃疡愈合质量,其机制可能与增强EGF、bFGF的表达和促进肉芽组织中微血管增生有关。壳聚糖与硫糖铝和雷尼替丁对提高溃疡愈合质量具有协同作用。
Background： Peptic ulcer is a common recurrent disease clinically. The ulcer recurrence is closely related to the quality of ulcer healing （QOUH）. Aims： To investigate the effect of chitosan on QOUH of gastric ulcer in rats and its possible mechanism. Methods： The rats were randomly divided into placebo group, two drug-control groups （sucralfate and ranitidine）, and three experimental groups （chitosan, chitosan with sucralfate and chitosan with ranitidine）. After 14 days treatment, the gastric ulcer tissues were collected to examine the size and surface area of the ulcer, and stained with HE, AB-PAS, factor VIII and SP immunohistochemical methods. A series of histological parameters and the expression of epidermal growth factor （EGF）, basic fibroblast growth factor （bFGF） and inducible nitric oxide synthase （iNOS） were detected. Results： In the experimental groups, the size of the ulcer decreased significantly more than that in the placebo and drug-control groups （P〈0.01）; the regenerated mucosal thickness, layers of glands, PAS-positive area and the number of microvessels in the granulation tissues increased remarkably than those in the placebo group （P〈0.01）, but the number of cystically dilated glands decreased （P〈0.01）. In the chitosan with sucralfate or with ranitidine groups, the number of cystically dilated glands was significantly lower than that in the ranitidine group （P〈0.05）, while PAS-positive area and the number of microvessels were higher （P〈0.05）; the layers of glands were significantly greater than that in the chitosan, sucralfate or ranitidine groups （P〈0.05）. The expression of EGF and bFGF in the three experimental groups were significantly higher than those in the placebo and ranitidine groups （P〈0.05）, while no significant difference was found in the expression of iNOS among all the groups. Conclusions： Chitosan can improve the QOUH of gastric ulcer in rats. Its mechanism maybe due to the increase of EGF, bFGF expression and the angiogenesis in the granulation tissues. Chitosan has synergistic effect with sucralfate and ranitidine in promoting QOUH.
出处
《胃肠病学》
2007年第3期148-152,共5页
Chinese Journal of Gastroenterology
基金
江西省卫生厅资助(No.02229)
