摘要
建立了A类大豆皂甙的高效液相色谱-差示折射(HPLC-dRI)检测方法。色谱条件为:ODS-AM-303色谱柱(YMC,4.6mm×250mm,5μm),柱温40℃,含0.1%三氟乙酸的乙腈-水(40∶60)为流动相,流速1mL/min。大豆皂甙A1和A2分别在1.39～6.96μg和1.67～8.34μg范围内线性关系良好,平均回收率为91.3%和92.6%,RSD为4.63%和3.48%。方法准确度高,重现性好,适用于大豆皂甙样品中A类皂甙的测定。
High -performance liquid chromatographic differential refractive index detective （HPLC-dRI） method was established to determine group A soyasaponins. The chromatographic conditions are as follows： ODS-AM-303 column （YMC, 4.6mm× 250mm, 5um） with the temperature of 40℃ , acetonitrile-water （40：60） containing 0.1% trifluoroacetic acid as mobile phase with the flow rate of 1mL/min. The method was proved to be linear in the range of 1.39-6.96ug and 1.67-8.34ug, and the recoveries were 91.3% and 92.6% with RSD of 4.63% and 3.48% for A1 and A2, respectively. The method is accurate and reproducible, suitable for the determination of group A soyasaponins.
出处
《食品工业科技》
CAS
CSCD
北大核心
2007年第3期209-211,共3页
Science and Technology of Food Industry
基金
国家自然科学基金项目(30360113)
