摘要
[目的]研究支架内部三维结构对β-TCP材料体内血管化的影响。[方法]将2种不同三维结构的β-TCP材料(孔径400～500μm,内连接径为120μm的圆片状β-TCP材料和相同成份、重量的β-TCP材料碎颗粒,直径在100～200μm)包埋入24只成年新西兰兔的双侧腰背筋膜内,术后1、2、4、8周对材料进行组织学观察、同位素骨扫描及扫描电镜等检查,观察2种不同内部三维结构β-TCP材料的体内血管化情况。[结果]两组材料具有良好的生物相容性。术后1周,2种结构材料只有周边部分孔隙内可见幼稚的毛细血管形成。术后4周,圆片状人工骨材料内全层出现新生血管,血管数量增多,管腔变大,外周血管发育成熟,进入血管化高峰期。术后8周血管数量无明显增多,仅有管腔增大,偶见成熟的微血管结构。而颗粒状材料血管化进程缓慢,血管数较少,管腔小,结构差。4周时,仍以发育幼稚的毛细血管为主,8周时出现部分毛细血管闭塞。[结论]材料孔隙间的连通是影响材料体内血管化的关键因素,具体地说,较高的连通率可以使材料血管化更为完全,而连通径的大小可以限制新生血管管腔的大小。
[ Objective] To study the role of scaffold internal three dimensional structure of on vascularization of β-TCP biomaterials in vivo. [ Method] Twenty four adult rabbits were selected for operation. Two different three-dimensional structure β- TCP biomaterials （wafer β-TCP, the pore diameter was from 400 μm to 500 μm, the pore inter- connection diameter was 120 μm; granulation β-TCP, the particle diameter was from 100 μm to 200 μm） were implanted separately into fascia lumbodorsalis of every rabbit. The specimens were harvested in 1, 2,4, 8 weeks after surgery for histology, scanning electronic microscope （SEM） and SPECT studies in order to observe the vascularization of two different structure β-TCP biomaterials in vivo. [ Result] The biocompatibility of two different β-TCP biomaterials was favourable. Only a few of immature blood capillaries were detected in some peripheral pores of two different biomaterials in one week after surgery. In four weeks of implantation,the result of histology indicated that the wafer artificial bone had vascularized completely. The number and lumens of blood vessel had increased. The peripheral blood vessel had been mature,showing vascularization crest-time. In eight weeks after sugery,there was no more increase of the number of blood vessel, while the lumens of blood vessel had increased. The mature capillaries were observed by chance. To compare with the wafer artificial bone,the vas cularization rate of the granulation artificial bone biomaterials was slower, and the number of blood vessel was less. On the other hand, the smaller lumens diameter and the infant structure existed in most of blood capillaries. Many blood vessels were not mature in four weeks,the vascular occlusion in some pores was detected. [ Conclusion] The pore interconnection pathway in scaffolds is a key factor for vascularization. In other words, the higher density of pore interconnection pathway can induct more complete vascularization in scaffolds,and the diameter can restrict the lumens of blood vessel diameter.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2007年第6期451-454,共4页
Orthopedic Journal of China
基金
国家自然科学基金资助项目(项目编号:50235020)
