摘要
目的探讨应用羟基磷灰石纳米材料和超液化碘油混悬液栓塞治疗,对肝癌组织表达CD147的影响。方法30只接种VX2肿瘤系的荷瘤兔随机分为3个治疗组:对照组;超液化碘油组;纳米材料和超液化碘油组。通过超选插管胃十二指肠动脉分别给予生理盐水(1mL/只),超液化碘油(0.3mL/kg),纳米材料和超液化碘油混悬液(0.3mL/kg)。术后3dCT检查确认插管成功。2周后,应用免疫组织化学法(S-P)和westernblot方法,来检测3组肿瘤组织CD147的表达。结果免疫组化显示,CD147在3组的肿瘤组织细胞膜均有表达,阳性率分别为(31.33±5.88)%,(75.63±4.44)%,(80.03±5.59)%,对照组与碘油组,纳米碘油组在CD147的表达差异有统计学意义(P<0.01),后两组差异则无统计学意义(P>0.05)。Westernblot半定量检测,碘油组和纳米碘油组CD147的表达量升高,与对照组对比有统计学意义(P<0.05)。结论应用超液化碘油,或羟基磷灰石纳米材料和超液化碘油混悬液栓塞治疗后,肿瘤组织表达CD147有上升趋势,可能是造成栓塞治疗后肿瘤复发率高的因素之一。
Objective To study expression of CD147 in rabbit VX2 tumor tissues after transarterial embolization with hydroxyapatite nanoparticles loaded with lipiodol. Methods Thirty rabbits implanted with tumor tissue of cell line VX2 were divided into three groups： control group, lipiodol group, and hydroxyapatite nanoparticles loaded with lipiodol group. The transarterial embolization was performed superselectively via gastroduodenal artery of rabbits, and each rabbit in control group was infused with 1 ml normal saline, that in lipiodol group was infused with 0. 3 ml lipiodol per kg, and o. 3 ml hydroxyapatite nanoparticles loaded with lipiodol per kg for that in the last group. Results of treatment were detected by using CT on day 3 after operation. After two weeks, three-step Immunohistochemical technique（ S-P ） and Western blot technique were used to investigate the expression of CD147 in tumor tissues of the 3 groaps. Results Immunohistochemieal results showed that CD147 was expressed on membrane of tumor cells in all three groups. The positive ratios of CD 147 in three groups were （ 31.33 ± 5.88 ） % , （ 75.63 ± 4.44 ） % , and （80. 03 ± 5. 59）% respectively, while the expression of CD147 in tumor tissues in control group was significantly different from others （ P 〈 0.01 ） , but for the other two groups, there was no difference between them（ P 〉0.05 ）. Protein half-quantitation detecting of CD147 with Western blot technique displayed that expression of CD147 in tumor tissues increased after embolization in last two groups as compared to that of control group （ P 〈 0.05 ）. Conclusions There was an increasing tendency expression of CD 147 in tumor tissues after transarterial embolization with hydroxyapatite nanoparticles loaded with lipiodol or only with lipigdol, and it may be one of the important factors for the high recurrence rate of hepatocellular carcinoma after embolization treatment.
出处
《中国普通外科杂志》
CAS
CSCD
2007年第3期213-217,共5页
China Journal of General Surgery
基金
国家"十五"科技攻关资助项目(2004BA714B08)
