摘要
利用利紫外差光谱、荧光光谱研究了3-甲基吲哚(粪臭素)与胰α-淀粉酶的相互作用。Stern2—Volmer淬灭曲线显示粪臭素对胰α-淀粉酶的荧光猝灭是静态猝灭。以lg[(F0-F)/F]对lg[Q]作线性拟合得出其结合常数KA=4.61×103(mol/L)-1,与结合位点数n=0.83。
The interaction beteen skatole and α-Amylase was studied by ultraviolet spectroscopy and fluorescence spectrometry. The Stern -Volmer plots showed that the quenching of skatole to α-Amylase was probably a static quenching process. The combination constant KA （4. 61 × 10^3 ）and the number of combination sites n （0. 83 ） was educed by graphical chart of lg [ （F0-F）/F] versus Ig [Q].
出处
《化学研究与应用》
CAS
CSCD
北大核心
2007年第4期405-408,共4页
Chemical Research and Application
