摘要
建立了毛细管电泳高频电导法测定琥乙红霉素的方法。探讨了缓冲溶液、有机溶剂添加剂以及分离电压和进样条件等因素对分离检测的影响。在电泳介质为2.0mmol/L柠檬酸-20.0%C2H5OH,分离电压20.0kV的优化条件下,在7min内即可实现琥乙红霉素的分离检测,线性范围为3.0μg/mL-150.0μg/mL,检出限为1.0μg/mL。方法简便、快速,可检测制剂中琥乙红霉素的含量。
A rapid method based on capillary electrophoresis with high frequency conductivity detection was described for the determination of Erythromycin ethylsuccinate. The key factors for separation and determination were studied and the best analysis conditions were obtained, in the buffer solution of 2. 0 mmol/L citric acid at the separation voltage of 20. 0 kV Erythromycin ethylsuccinate could be separated and detected within 7 min. Erythromycin ethylsuccinate showed good linear response in the range of 3.00mg/mL - 150. 0μg/mL, and the limit of detection was 1.0 μg/mL. The method is simple, quick, and suitable for the detec- tion of Erythromycin ethylsuccinate in tables and capsules.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2007年第4期437-440,共4页
Chemical Research and Application
基金
国家自然科学基金项目(20375049)
广东省科学技术项目(2004B10401027)
关键词
毛细管电泳
高频电导检测
非接触式电导检测
琥乙红霉素
capillary electrophoresis
high frequency conductivity detection
contactless conductivity detection
erythromycin ethylsuccinate
