摘要
目的探讨小儿系统性红斑狼疮(SLE)伴贫血的特征及其与肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ)和红细胞生成素(Epo)的相关性。方法采用ELISA法检测SLE患儿和正常儿童血清TNF-α、IFN-γ和Epo水平,观察TNF-α、IFN-γ对人红系集落形成单位(CFU-E)和红系瀑式集落形成单位(BFU-E)的影响及SLE患儿自身血清、Epo对SLE骨髓CFU-E的影响。结果与正常儿童相比,SLE伴贫血患儿血清TNF-α、IFN-γ和Epo均显著增高。TNF-α、IFN-γ抑制正常骨髓CFU-E和BFU-E集落形成,且随浓度增加,抑制作用增强。SLE自身血清能抑制其CFU-E形成。rhEpo能纠正TNF-α、IFN-γ和SLE自身血清对红系祖细胞的抑制。结论SLE患儿血清中存在造血干细胞抑制物;TNF-α、IFN-γ可能在贫血的发病中起作用;rhEpo可用于治疗SLE贫血。
Objective To elucidate the characteristics of children systemic lupus erythematosus （SLE） complicated with anemia, and the roles of gamma - interferon （ IFN - γ） , tumor necrosis factor -alpha （ TNF -α ） and erythropoietin （Epo）. Methods Serum levels of IFN - γ, TNF -α and Epo in SLE children patients and normal children were measured by enzyme linked immunosorbent assay （ELISA）. The influences of IFN - γ and TNF -α on colony forming unit - erythroid （ CFU - E） and burst forming unit - erythroid （ BFU - E）, and the influences of serum and Epo from SLE patients on CFU - E yields of SLE bone marrow cells. Results Serum levels of IFN - γ, TNF -α and Epo in SLE patients remarkably increased as compared with those in normal controls. IFN - γ and TNF - α inhibited CFU - E and BFU - E yields, and the effect increased following increases of concentrations, SLE antilogous serum inhibited CFU - E yield. Recombinant Human Erythropoietin （rhEPO） could correct the inhibition of erythroid progenitor cell by IFN - γ, TNF -α or serum from SLE patients. Conclusion There were hematopoietic inhibiting factors in the serum of SLE children patients. IFN - γand TNF -α might be involved in the pathogenesis of anemia in SLE patients. Administration of rhEpo might be beneficial for anemia in SLE patients.
出处
《中国全科医学》
CAS
CSCD
2007年第7期539-541,共3页
Chinese General Practice
