摘要
运用改良的血浆凝块体外培养方法，研究２４例成人原发性血小板减少性紫癜（ＩＴＰ）患者的骨髓在体外形成巨核细胞集落的能力。结果发现ＩＴＰ患者骨髓的巨核细胞集落生成率与正常对照无显著差异（ｐ＞０．２），其新鲜血清对自身骨髓巨核祖细胞（ＣＦＵ－ＭＫ）或异体正常骨髓ＣＦＵ－ＭＫ有不同程度的抑制作用（ｐ＜０．０１），血清经热灭活后这种抑制作用明显减弱。患者血清的抑制程度与其血清ＰＡＩｇＧ的量呈正相关。患者的血清免疫球蛋白Ｇ组份加补体对ＣＦＵ－ＭＫ生长有显著抑制作用（Ｐ＜０．０１），而完整的Ｔ淋巴细胞对ＣＦＵ－ＭＫ的生长有支持作用。
Megakaryocytopoiesis in 24 patients with idiopathic thrombocytopeic purpura(ITP)was investigated using a plasma clot culture assay.The number of bone marrow megakaryocyte colony forming cells(CFU-MK)has no difference between patients and controls(P<0.02).The unheated serum of patients has inhibiting effect on the bone marrow CFU-MK of patients and normal controls(P<0.01).But the heated serum has no obvious effects.The ability of inhibility is increased in relation to the amount of PAIgG in serum of patieht. The Serum IgG of ITP with complement had high activity for inhibiting the devecopment of CFU-MK,but the intact T lymphocytes of these patients supported the megakaryocytopoiesis.
出处
《南通医学院学报》
1996年第2期178-179,共2页
ACTA Academiae Medicinae Nantong
关键词
血小板减少性
紫癜
巨核祖细胞
体外培养
WT5”BZ〗Idiopathic thrombocytopenic purpura(ITP)
megakaryocyte colony forming cells(CFU-MK)
cell culture in vito
immunity
