摘要
以“中苜1号”紫花苜蓿7日龄无菌苗子叶和再生无菌苗的叶片为材料,建立了适用于农杆菌介导的转基因组织培养体系,并对MsNHX1基因进行转化。转化优化条件为:“中苜1号”紫花苜蓿7日龄无菌苗子叶、再生无菌苗叶片,用农杆菌菌液(A600=0.6)侵染6min,然后在培养基上铺一层灭菌滤纸培养7d后清洗,建立了苜蓿快速有效的遗传转化体系。
The regeneration system of somatic embryogenesis of genetic transformation for the cultivar alfalfa‘Zhongmu No. 1 ’ was established. Manipulation system was optimized by Kin--resistant calli assays and the histological detection of MsNHX1 activity, MsNHX1 gene corned from plant expression vector pBINHX. Two explants were infected with Agrobacterium tumefaciens （A600 = 0.6） for 8 rain and washed after 7 days of culture.
出处
《中国草地学报》
CSCD
2007年第2期102-106,共5页
Chinese Journal of Grassland
基金
"十五"国家高技术发展计划(863计划)(2002AA241101)
国家自然科学基金项目"紫花苜蓿盐胁迫特异表达基因的筛选与克隆"(30471110)
