摘要
目的应用微乳毛细管电泳法测定蟾酥及其制剂中蟾毒内酯类成分的含量。方法运行微乳缓冲液由0．81％正庚烷、6．61％正丁醇、3．31％SDS及89．27％10mmol／L硼砂溶液组成，pH：9．2。运行电压15kV（恒压分析）；柱温40℃；检测波长298nm。结果华蟾毒精和酯蟾毒配基的线性范围分别为54．8—548．0mg／L及51．6～516．0mg／L；平均回收率分别为97．29％-99．82％（RSD为1．86％～2．91％，n=6）及97．52％～101．69％（RSD为2．14％～3．02％，n=6）。结论该方法简便、准确、重现性好，可作为蟾酥及其制剂中蟾毒内酯类成分的质量控制方法。
Objective To establish a MEEKC method for determination of bufadienolides in toad venom and their Chinese medicinal preparations. Method The separation was achieved with 10mM sodium tetraborate （pH =9.2） containing n - heptane 0.81% , SDS 3.31% and n-butanol 6. 61% as buffer solution at a constant voltage of 15 kV and a temperature of 40 ℃. The detective wavelength was 298 nm. Results The linear determination range was 54.8 - 548.0 mg/L for cinobufagin and 51.6 - 516.0 mg/ L for recibufogenin . The average recoveries were 97.29% - 99.82% （ RSD 1.86 % - 2. 91% ,n = 6） for cinobufagin and 97.52% - 101.69% （ RSD 2.14% - 3.02, n = 6 ） for recibufogenin. Conclusion The method is simple and accurate with good reproducibility, and can be used for the quality control of bufadienolides in toad venom and their medical preparations.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2007年第3期203-205,共3页
Journal of Beijing University of Traditional Chinese Medicine
关键词
微乳毛细管电泳法
蟾酥
华蟾毒精
酯蟾毒配基
microemulsion capillary electrophoresis
toad venom
cinobufagin
resibufogenin
