摘要
为研究生育酚同系物的色谱分离过程,建立了考虑轴向扩散、液膜传质阻力和内扩散阻力的一般性速率模型,采用有限元和正交配置法求解该模型.模拟结果与实验数据相吻合,得到了液膜传质系数与流速的关系及孔内有效扩散系数,并将这些参数成功应用到色谱柱的放大过程中.采用正己烷-异丙醇体系分离了生育酚同系物,考察了流动相组成和流速对分离效果的影响.随着流动相中异丙醇体积分数的增大,生育酚同系物的容量因子和分离度减小.流动相中异丙醇体积分数以1%为最佳.
A general rate model, in which axial dispersion, liquid film mass transfer and effective diffusion in particles were taken into consideration, was adopted to investigate the chromatography process for tocopherols. The finite element and orthogonal collocation method were used to transform these equations to ordinary differential equations and to solve them. The simulation results agreed well with the experimental data. The relationship between the film mass-transfer coefficient and the interstitial velocity and the effective diffusion coefficient were measured. The scale-up of separation of the tocopherols from analytical to preparative scale was achieved. Total tocopherols were separated with n-hexane-isopropanol as the mobile phase using a silica-gel column. Effects of composition and the flow rate of the mobile phase on separation were investigated. As the concentration of isopropanol in the mobile phase increases, the capacity factor and the resolution decrease. The best concentration of isopropanol in the mobile phase is 1%.
出处
《浙江大学学报（工学版）》
EI
CAS
CSCD
北大核心
2007年第4期688-692,共5页
Journal of Zhejiang University：Engineering Science
关键词
液相色谱
一般性速率模型
生育酚
分离
liquid chromatography
general rate model
tocopherols
separation
