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丙型肝炎病毒核心抗原检测的临床应用价值 被引量:5

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摘要 目的将HCV核心抗原检测与HCV RNA PCR法和抗-HCV检测进行比较,评估HCV核心抗原检测的临床应用价值。方法采用ELISA法检测121份怀疑为HCV感染患者血清中的HCV核心抗原,并以HCV RNA PCR法和抗-HCV检测结果作为比较。结果121份样本中,PCR法测HCV RNA,阳性56例,阴性65例,ELISA法测HCV核心抗原,阳性52例,阴性69例,ELISA法测抗-HCV,阳性44例,阴性77例;HCV RNA PCR、HCV核心抗原、抗-HCV阳性率分别为46.28%、42.97%、36.36%。同HCV RNA PCR检测结果比较:HCV核心抗原检测符合率90.08%、假阳性率3.31%、假阴性率6.61%;抗-HCV检测符合率81.81%、假阳性率4.13%、假阴性率14.05%。结论HCV核心抗原检测与PCR检测的符合率较高。说明PCR法与HCV核心抗原检测都可以对抗-HCV出现前的感染进行早期诊断,从而缩短窗口期。
出处 《实用预防医学》 CAS 2007年第2期546-547,共2页 Practical Preventive Medicine
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  • 1崔晓红,潘卫,戚中田,宋艳斌,印凯,张晓华,陈汉,吴孟超.肝癌患者肝组织中丙型肝炎病毒的基因分型[J].中华医学杂志,1995,75(2):92-93. 被引量:8
  • 2易忠群,程新宪,赵怡生,江淑芳.丙型肝炎病毒抗原(HCVAg)检测及临床应用[J].陕西医学检验,1996,11(2):27-28. 被引量:1
  • 3陶其敏.HBsAg和HBeAg检测方法研究[J].北京医学院学报,1984,16:145-146.
  • 4戚中田,丙型肝炎病毒与丙型肝炎,1992年,162页
  • 5Nubling C M, Unger G, Chudy M, et al. Sensitivity of HCV core antigen and HCV RNA detection in the early infection phase[J]. Transfusion,2002 ,42(8):1037-1045.
  • 6idell A, Molnegren V, Pieksma F,et al. Detection of hepatitis C core antigen in serum or plasma as a marker of hepatitis C viraemia in the serological window-phase[J].Transfus Med,2002 ,12(2):107-113.
  • 7Pawlotsky JM. Use and interpretation of virological tests for hepatitis C. Hepatology 2002;36(5 Suppl 1):S65-S73.
  • 8Marcellin P, Asselah T, Boyer N. Fibrosis and disease progression in hepatitis C, Hepatology 2002;36(5 Suppl 1):S47-S56.
  • 9中华医学会肝病学分会.传染病与寄生虫病学芬会,丙型肝炎防治指南[J].中华肝脏病杂志,2004,12:194-198.
  • 10Saldanha J, Lelie N, Heath A. Establishment of the first international standard for nucleic acid amplification technology (NAT) assays for HCV RNA. Who collaborative study group.Vox Sang 1999;76:149-158.

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  • 1丙型肝炎防治指南[J].实用肝脏病杂志,2004,7(3). 被引量:38
  • 2丙型肝炎防治指南[J].临床肝胆病杂志,2004,20(4):197-203. 被引量:735
  • 3欧阳奕,谭德明,李铁刚,周辉,谭畅.慢性丙型肝炎患者血清中HCV核心抗原的定性检测[J].中南大学学报(医学版),2006,31(6):894-896. 被引量:8
  • 4Medhi S, Potukuchi SK, Polipalli SK. et al. Diagnostic utility of hepatitis C virus core antigen in hemodialysis patients[J]. Clin Biochem,2008,41 (7-8):447-452.
  • 5Widell A,Molnegren V,Pieksma F,et al. Detection of hepatitis C core antigen in serum or plasma as a marker of hepatitis C viraemia in the serological windowphase [J]. Trandfus Med,2002,12(2) : 107-113.
  • 6Aoyagi K, Ohue C, Iida K, et al. Development of a simple and highly sensitive enzyme immunoassay for hepatitis C virus core antigen[J]. J Clin Microbiol, 1999,37(6):1802- 1808.
  • 7Gaudy C,Thevenas C,Tichet J,et al. Usefulness of the hepatitis C virus core antigen assay for screening of a population undergoing routine medical checkup[J]. J Clin Microbiol, 2005,43 (4) : 1722-1726.
  • 8Peterson J, Green G, Iida K, et al. Detection of hepatitis C core antigen in the antibody negative "window"phase of hepatitis C infection [ J ]. Vox Sang, 2000,78 : 80-85.
  • 9Barrera JM, Francis B, Ercilla G, et al. Improved detection of anti-HCV in post-transfusion hepatitis by a third-generation ELISA [ J ]. Vox Sang, 1995,68 : 15-18.
  • 10Busch MP, Rawal BD, Nowicki M,et al. HCV RNA and ALT patterns in seroconverting transfusion recipients, and correlation with donor ALT and RNA [J]. Transfusion, 1999,37(supp1) : 111S.

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