摘要
[目的]观察化瘀通络汤对大鼠实验性溃疡性结肠炎(UC)的疗效和对其血小板活化的影响,探讨化瘀通络汤治疗UC的机制及UC的发病机制。[方法]将动物按体重随机分为化瘀通络组、柳氮磺胺吡啶(SASP)组、模型对照组和正常对照组。其中除正常对照组外其余3组均采用2,4-二硝基氯苯和醋酸复合法制作UC大鼠模型。各组连续治疗4周后,取外周血测血清P-选择素和可溶性CD40配体(sCD40L)的水平。[结果]模型对照组血清P-选择素和sCD40L水平及肠黏膜损伤评分较正常对照组、化瘀通络组和SASP组显著升高(P<0.05),而化瘀通络组与SASP组间差异无统计学意义(P>0.05)。[结论]血小板活化在UC发病过程中起重要作用。化瘀通络汤治疗UC有效,其机制可能在于对血小板活化的阻抑而间接对免疫炎症进行调解,也可能直接通过对免疫炎症反应的调解而实现。
[Objective]To observe the effect of Huayutongluo decoction （HYTLD） on platelet activation in rat experimental ulcerative colitis（UC） and investigate the mechanism of HYTLD treating rat experimental UC. [Methods]Rat experimental ulcerative colitis model was induced by the combination of dinitro-chlorobenzene and acetic acid. After being treated for 4 weeks , the index of mucosal damage was observed, the concentration of soluble CD40 ligand （sCD40L） and P selectin in rats＇ serum was detected by ELISA. [Results] Compared with model controls, serum P selectin and sCD40L levels were significantly lower in HYTLD group, SASP group and normal group（P〈0. 05）. There was no significant difference between SASP group and HYTLD group（P〉0.05）. [Conclusion]The platelet activation plays important role in pathogenesis of UC. HYTLD is an effective decoction to treat rat experimental UC. The mechanism may be by depressing platelet activation and adjusting immune inflammation.
出处
《中国中西医结合消化杂志》
CAS
2007年第2期81-84,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
全军"十一五"重点课题基金资助项目(06MA049)
关键词
结肠炎
溃疡性
化瘀通络汤
血小板活化
ulcerative colitis
Huayutongluo decoction
platelet activation
