摘要
目的通过借助Histidine Tryptophan Ketoglutarate（HTK）保护液在肾移植模型中的器官保护作用，研究肾移植缺血再灌注对热休克蛋白（HSP）72表达的影响。方法18只实验用Sprague Dawley（SD）大鼠随机分为2组，分别进行肾移植。单纯冷藏组（C组）：供体肾脏摘除后，在4℃温度下置于普通Ringer solution中保存10h后进行肾移植；HTK组（H组）：供体肾脏摘除后，在4℃温度下置于HTK solution中保存10h后进行肾移植；肾移植缺血再灌后6h，测定血清尿素氮（BUN）、血清肌酐（Cr）、乳酸脱氢酶（LDH）和天冬氨酸氨基转移酶（AST）；组织样本用于HSP72表达指数（HI）的测定，以了解热休克蛋白表达对肾移植中缺血再灌注损伤的评价作用。结果H组血清BUN、Cr、LDH、AST明显低于C组（P〈0．05）；HSP72的表达H组明显高于C组（P〈0．01）。结论HTK保护液对肾脏有明显的保护作用；缺血再灌注后热休克蛋白的表达标志着细胞的再生，并具有一定程度的内源性保护作用。
Objective To study the heat-shock protein 72（HSP72） on the protection of kidney in transplantation ischemic reperfusion injury by use of histidine tryptophan ketoglutarate（HTK）. Methods 18 SD rats were randomly divided into 2 groups, which underwent kidney transplantation. The donor＇s kidney was cold stored in 4 ℃ normal Ringer solution for 10 hours, and then the implantation was performed in group C ;Tthe donor＇s kidney was cold stored in 4 ℃ HTK solution for 10 hours in HTK group （H） , and then the implantation was performed; 6 hours after reperfusion, blood and tissue samples were collected for testing serum BUN, creatinine （ Cr）, LDH, AST, and HSP72 expression index （HI）. Results Serum BUN,Cr, LDH, and AST in H group all showed significant lower levels than in C group （ P 〈 0.05 ） ; HI in H group was significantly higher than in C group （ P 〈 0.01 ）. Conclusion HTK solution has a protective effect on renal graft ; HSP72 is a good indicator of kidney recovery and also has certain protective role.
出处
《中国综合临床》
北大核心
2007年第5期453-455,共3页
Clinical Medicine of China
关键词
肾移植
HTK保护液
热休克蛋白72
Kidney transplantation
HTK preservation solution
Heat-shock protein 72
