摘要
目的:观察温下方对Lewis肺癌的抑制作用及对细胞周期的影响。方法:C57BL/6小鼠皮下接种Lewis肺癌,随机分组,口服给药后计算抑瘤率、脾及胸腺指数;流式细胞技术分析肿瘤细胞周期变化及凋亡百分率。结果:温下方质量分数在生药2.8、5.57和11g/(kg.d)时,对小鼠Lewis肺癌的抑制率分别为31.0%、34.3%和37.6%;肺癌细胞凋亡率增加至(11.13±4.52)%、(16.31±6.12)%和(20.08±5.13)%;G0/G1期细胞比例增加至(50.40±5.37)%、(56.01±5.91)%和(62.38±5.22)%,呈明显的剂量依赖关系。结论:温下方抑制肿瘤生长的机制可能与阻滞细胞周期、诱导细胞凋亡有关。
OBJECTIVE： To study the effects of Wenxiafang, a traditimal Chinese herbal fomula, on C57BL/6 mouse of Lewis. METHODS： The transplantation tumor model was established by hypo dermic inoculation of Lewis lung cancer, the inhibit-rate and spleen and thymus index were calculated after the randomization and administera tion of the drug; the cell cycle and ceil apotosis were evaluated by the fluor escenee technique and flow cytometry. RESULTS： The carcinomw inhibiting rates of Wenxiafang were 31.0%, 34. 3% and 37.6%, the apoptotic rate of lung cells increased to （11.13± 4. 52）%, （16.31 ± 6. 12）% and （20. 08±5.13）%, the percentage of cells in G0/G1 increased to （50.40 ± 5.37）%, （56.01± 5.91）% and （62.38 ±5.22）%, respectively when the drug was given at the doses of 2.8, 5.57, and 11 g/（kg·d）,in a dose-dependent way. CONCLUSION： Wenxiafang might inhibit tumor proliferation by blocking cell-cycle and inducing cell apoptosising.
出处
《中华肿瘤防治杂志》
CAS
2007年第5期339-341,共3页
Chinese Journal of Cancer Prevention and Treatment
基金
国家教育部博士点基金(20050441007)
