摘要
目的:观察大黄素对人肺腺癌A-549细胞增殖及周期的影响。方法:设计空白对照组及不同浓度的大黄素溶液作用于A-549细胞,MTT比色法测定A-549细胞生长的抑制率;流式细胞仪分析A-549细胞增殖周期的变化。结果:大黄素对人肺腺癌A-549细胞有明显的生长抑制作用,其抑制作用呈浓度依赖性,其抑制率随药物浓度升高而增加,400mg/L时达到83.33%,实验组与对照组之间差异有统计学意义,P=0.000;对A-549细胞具有细胞周期阻滞作用,可将其阻滞于G0/G1期,阻止细胞进入S期。结论:大黄素对人肺腺癌A-549细胞的增殖生长具有显著的抑制作用;并可使A-549细胞增殖周期停滞于G0/G1期,而阻止其进入S期。
OBJECTIVE： To investigate the effect of emodin on the cell proliferation periods of human lung adenoeareinoma cell line A-549. METHODS： A-549 cells were divided into control group and emodin groups. The growth restrain rate of the A-549 ceils was deter mined by MTT, and the cell proliferation periods of the A-549 cells were analysed. RESULTS： Emodin had obviously the function of the restrain of the A549 cell growth, the function intension dependented on the density of emodin, the growth restrain rate of the A-549 cells was along with the density of emodin, and it was 83.33% when density of emodin was 400mg/L. Through repeate measure analysis of variance, it was found that there was a significant difference between the experi ment groups and control group, P=0. 000. Emodin had the function of block cell peridos on A-549, it stoped the A-549 cells at G0/GL period. CONCLUSION： Emodin has obviously the function of the restrain of the A-549 cell growth, but also has tbe function of the block cell peri dos on A-549, it can stop A-549 cells at G0/G1 period.
出处
《中华肿瘤防治杂志》
CAS
2007年第5期342-344,共3页
Chinese Journal of Cancer Prevention and Treatment
基金
陕西省中医管理局科研基金(2003029)
关键词
大黄素
肺肿瘤
细胞增殖
细胞周期
emodin
lung neoplasms
cell proliferation
cell cycle
