摘要
目的:探讨端粒酶逆转录酶(TERT)的反义寡核苷酸(ASODN)对甲状腺癌(TC)细胞凋亡的影响.方法:体外培养TC细胞,TERTASODN和正义寡核苷酸(SODN)分别作用于体外培养的TC细胞,采用透射电镜,Hoechest荧光染色,流式细胞仪等方法分析ASODNTERT对TC细胞凋亡的影响.结果:透射电镜示ASODN可诱导TC出现典型的细胞凋亡形态,Hoechest荧光染色显示ASODN可使TC凋亡数目增多从52±16上升到178±32,流式细胞仪证实5μmol/LASODN处理组,SODN处理组及空白对照组的细胞凋亡率分别为10.4%,1.6%和无凋亡(P<0.05).结论:TERTASODN能增强TC细胞凋亡.
AIM： To investigate the effect of antisense oligonu- cleotides （ASODN） encoding telomerase reverse transcriptase （TERT） mRNA on thyroid carcinoma （TC） cell apoptosis. METHODS： The ASODN and sense oligonucleotides （SODN） encoding TERT were delivered respectively to the TC cells in vitro. The effect of ASODN encoding TERT mRNA on TC cell apoptosis was detected by transmission electron microscope, Hoechest 33258 staining and flow cytometry. RESULTS： Transmission electron microscope showed ASODN could induce typical apoptotic manifestations of TC cells; Hoechest 33258 staining showed ASODN could increase the number of apoptotic cells from 52 ± 16 to 178 ± 32; flow cytometry revealed that the apoptosis rates of TC cells treated by 5 umol/L ASODN, SODN, or nothing were 10.4% , 1.6% and 0, respectively （ P 〈 0. 05 ）. CONCLUSION： ASODN complementary to TERT mRNA at some concentration can sequence-specifically enhance TC cell apoptosis.
出处
《第四军医大学学报》
北大核心
2007年第7期624-626,共3页
Journal of the Fourth Military Medical University
关键词
甲状腺肿瘤
寡核苷酸类
反义
端粒酶逆转录酶
thyroid neoplasms
oligonucleotides, antisense
telomerase reverse transcriptase
