摘要
目的:研究苦参碱(matrine,MAT)对人卵巢恶性畸胎瘤细胞株PA-1体外增殖及诱导凋亡的影响。方法:MTT法检测苦参碱对细胞增殖的抑制的量效和时效关系;二脒基苯基吲哚(DAPI)染色检测细胞形态学变化;流式细胞仪检测细胞凋亡和细胞周期。结果:MTT法显示苦参碱有明显的抑制增殖作用,且呈时间依赖性和剂量依赖性;DAPI染色可见给药组出现明显核固缩且呈剂量依赖性;流式细胞仪检测1.0mg/ml、0.5mg/ml、0.25mg/ml浓度的苦参碱作用24h后PA-1细胞的凋亡率依次下降;有明显的G1期阻滞作用,且具有剂量依赖性。结论:苦参碱能通过诱导凋亡、产生G1期阻滞显著抑制PA-1细胞的增殖,且凋亡率具有剂量依赖性。
Objective：To study the effects of matrine on proliferation in vitro and apoptosis of PA-1 human ovary malignant teratoma cell line. Methods： The inhibition ability of matrine on the cell line was detected with MTT assay and the dose-effect relationship and the time-effect relationship were found. The morphological changes of the cells with DAPI dyeing was observed. Apoptosis rates and cell cycle were detected with flow cytometry. Results： The inhibition rates of PA-1 cells were decreased according to matrine＇s concentration and time. While observed by DAPI dyeing, there was obviously karyopycnosis in the cells. The result of flow cytometry showed that matrine could induce apoptosis of PA-1 cells, stop the growth of them at G1 period and restrain mitosis of cells.And the apoptosis rates of 1.0,0.5,0.25 mg/ml concentration of matrine were declined in order after they affected the cells for 24 hours. Conclusion： Matrine can inhibit the growth of PA-1 cells through inducing apoptosis. The apoptosis effect is dose-dependent.
出处
《温州医学院学报》
CAS
2007年第2期97-99,共3页
Journal of Wenzhou Medical College
基金
浙江省自然科学基金资助项目(Y204463)
浙江省卫生厅卫生科技重点科研基金资助项目(2004ZD009)。
