摘要
将BMP-2腺病毒载体(Ad-BMP-2)与纤维蛋白凝胶混合后复合PLA／PCL(聚乳酸／聚己内酯)支架,移植修复骨缺损。于新西兰大耳白兔双侧桡骨中段造成1．5 cm骨缺损,采用四种方法进行处理:A组:Ad-BMP-2+纤维蛋白凝胶+PLA／PCL;B组:重组BMP-2+纤维蛋白凝胶+PLA／PCL;C组:对照基因(Ad-Lacz)+纤维蛋白凝胶+PLA／PCL;D组:纤维蛋白凝胶+PLA／PCL。结果表明,术后12周A组缺损区在成骨活跃程度、骨再生量和再生髓腔结构等方面均显著优于B组,其骨缺损得到了较彻底的修复。C、D两组均不能产生骨性愈合。PLA／PCL协同纤维蛋白凝胶运载BMP-2基因修复节段性骨缺损可达到较好的效果。
Adenovirus carrying BMP-2 gene, after being mixed with fibrinous gel, was siphoned off on biodegradable scaffolds （PLA/PCL）. The composite was used to repair 1.5 cm long radius defect in rabbits. Four methods were in use in the experiments： Ad-BMP-2 plus fibrinous gel and PLA/PCL （Group A）, reconstructed hBMP-2 plus fibrinous gel and PLA/PCL （Group B）, Ad-Lacz plus fibrinous gel and PLA/PCL （Group C）, and fibrinous gel and PLA/PCL （Group D）. Results showed that the defects treated in Group A were repaired with much more new bone regenerated, bridged earlier and stronger than those in Group B 12 weeks after operation. The defects treated in the other two groups could not attain osseous tissue healing. BMP-2 gene carried by biodegradable scaffold and fibrinous gel is easy to conduct and has very strong osteoinduction ability. It is really a good method to repair segmental bone defects.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2007年第2期345-349,共5页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(39800151)
关键词
骨形态发生蛋白2
基因治疗
组织工程
骨愈合
Bone morphogenetic proteins-2 （BMP-2） Gene therapy Tissue engineering Bone healing
