摘要
目的比较研究大鼠颌骨及胫骨骨再生修复过程中不同时期同源异型盒基因Hoxa2(homeoboxa2)表达,探讨骨再生修复的分子调控机制。方法建立SD大鼠一侧颌骨及胫骨骨缺损模型,另一侧为正常组织对照,分别于术后3、7、10、14、21 d提取缺损处骨痂及对照骨组织总RNA,以Hoxa2 mRNA第636-880碱基序列设计引物,β-actin为内参,采取RT-PCR扩增,检测Hoxa2 cDNA表达。结果所有实验组及对照组胫骨组织均扩增出片段长度为245 bp的目的基因条带,为Hoxa2 cDNA基因片段,半定量PCR分析发现实验组较对照组表达增强(P<0.05);所有实验组及对照组颌骨组织均未扩增出Hoxa2 cDNA目的基因条带。结论与其在胚胎发育过程中的表达特点相似,Hoxa2在颌骨再生修复中不表达,而在胫骨骨再生修复中表达增强,提示颌骨再生修复可能存在独特的分子调控机制。
Objective To investigate the mechanism of molecular regulation of bone regeneration, and the expression of homeobox a2 （Hoxa2） gene in the different periods of jaw and tibia bone repair. Methods The SD rat model of jaw and tibia bone defect was constructed in one side,and the other side was taken as control group. Total RNA was respectively extracted from calluses on day 3,7, 10, 14 and 21 after the operation, as well as control group. Primers were designed by 636th-880th base sequence of Hoxa2 mRNA and β-actin made inside reference. The expression of Hoxa2 was examined by RT-PCR amplification. Results The gene fragment of Hoxa2 cDNA, whose length was 245 bp, was detected both in the repair group and control group in tibia bone. However, the Hoxa2 cDNA was not detected in the jaw bone tissue. Conclusions During adult bone regeneration, hoxa2 was expressed in the tibia, but not in the jaw bone, which was similar to embryonic bone development. It was suggested the regeneration of the jaw bone may have a distinctive mechanism of molecular regulation.
出处
《口腔医学》
CAS
2007年第4期193-195,共3页
Stomatology
基金
江苏省自然科学基金资助项目(BK2006173)
