摘要
目的定量检测传染性单核细胞增多症(传单)患儿外周血EBV-DNA含量,并探讨其临床意义。方法采用荧光定量PCR方法检测82例传单患儿和40例健康儿童外周血EBV-DNA含量,并进行统计分析。结果传单组EBV-DNA的阳性率为84.14%(69/82),对照组阳性率为5.00%(2/40),阳性率差异有显著性(P<0.01);传单患儿外周血早期EBV-DNA含量与恢复期比较差异有显著性(P<0.01)。结论荧光定量PCR方法检测外周血EBV-DNA含量对传单的诊断有极高价值,可作为判断疗效及监测病情的一种有效手段。
Objective To determine the content of Estein-Barr virus （EBV） DNA in peripheral blood of children with infectious mononucleosis （IM） and explroe its clinical significance. Methods EBV- DNA in peripheral blood of 82 cases with IM and 40 cases of healthy control were detected by Fluorescent Quantitative PCR. Results rllae positive rate of EBV- DNA in IM group was 84.14% （69/82） and 5.0% （2/40） in the control group, the percent of positive in the former was significant higher than the later （ P 〈 0.01 ）. EBV - DNA concentration of patient with IM in early stage was higher than 1 ater stage （ P 〈 0. 01 ）. Conelusion Quantitative analysis of peripheral blood EBV - DNA may he a valuable technique for diagnosing patients with IM and predicting the prognosis.
出处
《中国热带医学》
CAS
2007年第5期677-678,共2页
China Tropical Medicine
