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Twist在人肝癌细胞系与正常肝细胞系中的表达差异 被引量:5

Up-regulated expression of Twist gene in human hepatocellular carcinoma cell line
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摘要 目的研究Twist在人肝癌细胞系HepG2和人正常肝细胞系L02中的表达及其作用。方法利用荧光定量PCR技术及免疫印迹法分别检测TwistmRNA及Twist蛋白在人肝癌细胞系HepG2及人正常肝细胞系L02中表达的差异。结果HepG2细胞的TwistmRNA表达水平为L02的2.36倍(P<0.05),蛋白水平为(3.13±0.21)倍(P<0.05)。结论Twist在肝癌细胞系中的过高表达可能与肝癌的发生、发展有关。 Objective To study the differential expression of Twist gene in human hepatoma cell line HepG2 and human hepatic cell line L02. Methods By using real time PCR and Western blotting, the expression of Twist mRNA and protein in HepG2 and L02 was detected respectively. Results The expression of Twist mRNA in HepG2 was 1.36 fold higher than that in L02, and that of Twist protein in HepG2 was(3.13 ± 0. 21)fold higher than that in L02. Conclusion The overexpression of Twist in hepatic cancer cell line might be related to the progression of hepatocellular carcinoma.
出处 《腹部外科》 2007年第2期115-117,共3页 Journal of Abdominal Surgery
基金 卫生部临床重点学科资助项目
关键词 肝肿瘤 聚合酶链反应 免疫印迹法 Liver neoplasms Polymerase chain reaction Immunoblotting
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  • 1Kountouras J,Zavos C,Chatzopoulos D.Apoptotic and anti-angiogenic strategies in liver and gastrointestinal malignancies.J Surg Oncol,2005,90:249-259.
  • 2Thisse B,Messal M,Perrin-Schmitt F.The twist gene:Isolation of a drosophila zygotic gene necessary for the establishment of dorsoventral pattern.Nucleic Acids Res,1987,15:3439-3453.
  • 3Maestro R,Dei Tos AP,Hamamori Y,et al.Twist is a potential oncogene that inhibits apoptosis.Genes Dev,1999,13:2207-2217.
  • 4Martin TA,Goyal A,Watkins G,et al.Expression of the transcription factors snail,slug and twist and their clinical significance in human breast cancer.Ann Surg Oncol,2005,12:488-496.
  • 5Jing Y,Mani SA,Donaher JL,et al.Twist,a master regulator of morphogenesis,plays an essential role in tumor metastasis.Cell,2004,117:927-939.
  • 6Rosivatz E,Becker I,Specht K,et al.Differential expression of the epithelial-mesenchymal transition regulators snail,SIP1 and twist in gastric cancer.Am J Pathol,2002,161:1881-1891.
  • 7Kwok WK,Ling MT,Lee TW.Up-Regulation of twist in prostate cancer and its implication as a therapeutic target.Cancer Res,2005,65:5153-5162.
  • 8Desprez PY,Sumida T,Coppe JP.Helix-loop-helix proteins in mammary gland development and breast cancer.J Mammary Gland Biol Neoplasia,2003,8:225-239.
  • 9Chen ZF,Behringer RR.Twist is required in head mesenchyme for cranial neural tube morphogenesis.Genes Dev,1995,9:686-699.
  • 10Kang Y,Massague J.Epithelial-mesenchymsl transitions:Twist in development and metastasis.Cell,2004,118:277-279.

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