摘要
利用利迪链霉菌生防菌株A02发酵液和番茄灰霉病菌对番茄植株进行诱导和接种处理,利用分光光度法测定处理前和处理后1,5d番茄叶片组织的主要防御酶系——苯丙氨酸解氨酶、过氧化物酶、过氧化氢酶和多酚氧化酶的活性变化。试验结果显示,不接种灰霉病菌而单独诱导处理、接种灰霉病菌后诱导处理和诱导处理后接种灰霉病菌这3种处理都能提高番茄植株各防御酶系的活性,后者酶活性峰值最高,对番茄植株灰霉病的抑制作用也最明显,控制效果达94.92%。
The study on inducing resistant effect of Streptomyces lydicus A02 to tomato gray mold were carried out in greenhouse. Tomato seedlings were treated via three methods: only inducing with fermentation broth of cus A02, inducing after inoculating with spore suspension of Botrytis cinerea, inoculating after inducing. The activities of 4 defense enzymes in tomato leaves were assayed by ultraviolet-visible absorption spectroscopy. The results showed that all the three treatments could increase the activities of defense enzymes, of which the treatment of inoculating after inducing presented a better inducing effect on 4 defense enzymes and a better control effect on tomato gray mold than inducing after inoculating did. The control effect was up to 94.92 %.
出处
《华北农学报》
CSCD
北大核心
2007年第2期152-155,共4页
Acta Agriculturae Boreali-Sinica
基金
北京市科技计划项目(D0706005000091)
国家自然科学基金资助项目(30671346)
北京市自然科学基金项目(6072010)