摘要
研究了以索尔邦百合(Lilium Sorrbonne)的鳞片叶为外植体的组培和植株离体再生技术,培养基采用MS基本培养基,附加不同的激素组合。分别获得诱导的愈伤组织、芽及根,并遴选出最佳激素组合,以确立索尔邦百合组织培养再生系统。试验结果表明适合的诱导培养基是MS+BA0.5mg/L+NAA0.2mg/L;继代培养基为MS+2,4-D1.0mg/L和MS+BA 0.5mg/L +NAA0.2mg/L;生根培养基是1/2MS+IBA0.2mg/L和1/2MS+NAA0.2mg/L。该方法最大增殖倍数达到5.74。
The organogenesis and plant regeneration of Lilium Sorbonne was studied by using the segments of bulb as initial explants, The calli, sprouts and roots were induced respectively on the various media got by adding different hormone to MS basic medium. The optimum hormone combinations were selected and plant regeneration system was established The results showed the optimal medium for explant induction was MS＋BA0. 5 mg/L A-NAA 0. 2 nag/L, optimal medium for proliferating clump sprouts was MS＋2,4-D 1. 0 mg/L or MS＋BA 0. 5 mg/L ＋NAA0. 2 mg/L , and the optimal medium for the plantlet rooting was 1/2MS＋NAA0. 2 mg/L or 1/2MS＋IBA0. 2 mg/L. The result showed that the propagation coefficient reached 5.74
出处
《北方园艺》
CAS
北大核心
2007年第4期212-214,共3页
Northern Horticulture
基金
江苏省博士后基金(0208003402)
关键词
索尔邦百合
组织培养
快繁
Lilium Sorbonne
Tissure culture
Rapid propagation
