摘要
研究了苯酚、Cu2+、亚硝酸盐和总氨氮对8~12日龄中华鲟Acipenser sinensis稚鱼的急性毒性,中华鲟稚鱼的全长为(2.36±0.10)cm,平均体重为0.07 g。结果表明:苯酚对中华鲟稚鱼24、48、72 h和96h的半致死浓度(LC50)及95%可信区间分别为97(87~109)、83(73~95)、75(69~83)mg/L和71(64~80)mg/L;Cu2+对中华鲟稚鱼的24、48、72 h和96 h的LC50及95%可信区间分别为0.31(0.29~0.34)、0.20(0.15~0.27)、0.09(0.08~0.12)mg/L和0.07(0.06~0.10)mg/L;亚硝酸氮对中华鲟稚鱼的24、48、72 h和96 h的LC50及95%可信区间分别为7.05(6.22~8.03)、4.07(1.51~7.88)、3.10(2.07~4.34)mg/L和2.57(2.31~2.85)mg/L;非离子氨对中华鲟稚鱼的24、48、72 h和96 h的LC50及95%可信区间分别为0.67(0.64~1.07)、0.52(0.44~0.67)、0.43(0.40~0.54)mg/L和0.39(0.30~0.45)mg/L。126 mg/L苯酚浓度组中,中华鲟稚鱼的心率(119±10次/min,n=10)明显快于对照组(106±5次/min,n=10)(P<0.05);0.4 mg/L Cu2+浓度组中,稚鱼的心率与对照组没有显著差别;20.30 mg/L亚硝酸氮和29.20 mg/L总氨氮浓度组中,鱼的心率分别为(112±4)次/min和(112±7)次/min,也显著高于对照组(P<0.05)。
Acute toxicities of phenol, Cu^2＋ , nitrite and total ammonia to juvenile Chinese sturgeon Acipenser sinensis fingerlings were determined in static bioassays. The juveniles with （2.36±0.10 ）cm in total length and average 0.07 g in body weight were 8 - 12 day old just before the yolk would be completely exhausted. It was found that the 24, 48, 72 h and 96 h median lethal concentration （LCso） values （95% confidence interval ） of the phenol to the juveniles were 97 （87 -109）,83 （73 -95 ）, 75 （69-83 ）mg/L and71 （64-80 ）mg/L, respectively. The 24, 48, 72 h and 96 h LCso values （95% confidence interval ） of Cu^2＋ to the juveniles were 0.31 （0.29 -0.34）, 0.20 （0.15 -0.27 ）, 0.09 （0.08 -0.12 ）mg/L and 0.07 （0.06 -0.10 ）mg/L, respectively. The24 , 48, 72 h and 96 h LCso values （95% confidence interval ） of nitrite - N to the juveniles were 7.05 （6.22 - 8.03 ）, 4.07 （1.51 -7.88 ）, 3.10 （2.07 -4.34 ）mg/L and 2.57 （2.31 -2.85 ）mg/L, respectively. The 24, 48, 72 h and 96h LCso values （95% confidence interval ） of unionized ammonia - N to the juveniles were 0.67 （0.64 - 1.07）, 0.52 （0.44-0.67）, 0.43 （0.40-0.54） mg/L and 0.39 （0.30-0.45）mg/L, respectively. The juveniles had higher heart beating rates （ 119 ＋ 10 beat/min, n = 10） in 126 mg/L phenol than those in the control group （106±5 beat/min, n = 10） （ P 〈 0.05）. There was no significant difference in heart raites in 0.4 mg/L Cu^2＋ and in the control group（ P 〉 0.05）. The juveniles had significantly higher heart beating rates in 20.30 mg/L nitrite - N （ 112 ±4 beat/min, n = 10） and 29.20 mg/L total ammonia - N （ 112±7 beat/min, n = 10） than in the control group （ P 〈 0.05 ）.
出处
《大连水产学院学报》
CSCD
北大核心
2007年第2期118-122,共5页
Journal of Dalian Fisheries University
基金
国家自然科学基金资助项目(30490231)
科技部基础性工作重点专项项目资助(2002DEA1004)
中国长江三峡工程开发总公司项目
国务院三峡办重点项目资助(SX97-25/HB)
关键词
中华鲟
急性毒性
半致死浓度
苯酚
CU^2+
亚硝酸盐
总氨氮
Acipenser sinensis
acute toxicity
median lethal concentration
phenol
Cu^2＋
nitrite
total ammonia
