摘要
Photo-induced Fenton reaction was utilized in this paper to control the sizes of polyethylenimine hydrogels （M-PEIs,M,Fe atom） as gene delivery vector.H2O2 and FeCl2 were mixed with PEI prepolymer in water in a 3-neck quartz flask, and the mixture was stirred vigorously.M-PEIs nanogels dispersed very well in water could be prepared under illumination by use of low-pressure Hg lamps at room temperature.This system was protected from oxygen with N2.By changing reaction conditions,such as the pH value,reaction time and concentrations of solutes,sizes of M-PEIs nanogels could be controlled.It was found that the sizes of M-PEIs decreased with the pH value and the concentrations of FeCl2.The increasing of the initiator （H2O2） concentrations would make the particles more tighten,but might result in crosslinking or degradation of particles.A suitable irradiation time ensured formation of homogeneous nanogel products,but prolonged irradiation would cause degradation of nanogels.After photo crosslinking,the products were purified with membrane filters of 0.45 μm and treated further by dialysis bags with size exclusion below 10 kDa in order to make them more stable for storage.
Photo-induced Fenton reaction was utilized in this paper hydrogels （M-PEIs, M, Fe atom） as gene delivery vector. H2O2 and FeCl2 in a 3-neck quartz flask, and the mixture was stirred vigorously. M-PEIs to control the sizes of polyethylenimine were mixed with PEI pre-polymer in water nanogels dispersed very well in water could be prepared under illumination by use of low-pressure Hg lamps at room temperature. This system was protected from oxygen with N2. By changing reaction conditions, such as the pH value, reaction time and concentrations of solutes, sizes of M-PEIs nanogels could be controlled. It was found that the sizes of M-PEIs decreased with the pH value and the concentrations of FeCl2. The increasing of the initiator （ H2O2 ） concentrations would make the particles more tighten, but might result in crosslinking or degradation of particles. A suitable irradiation time ensured formation of homogeneous nanogel products, but prolonged irradiation would cause degradation of nanogels. After photo crosslinking, the products were purified with membrane filters of 0.45 μm and size exclusion below 10 kDa in order to make them more stable for storage. treated further by dialysis bags with size exclusion below 10 kDa in order to make them more stable for storage.
出处
《高分子学报》
SCIE
CAS
CSCD
北大核心
2007年第4期389-393,共5页
Acta Polymerica Sinica
基金
上海市纳米专项(项目号0243nm027)
中科院上海应用物理研究所前沿领域(项目号55120212)资助
