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胞外多糖和脂多糖在青枯菌对尾巨桉根部吸附和侵入过程中的作用研究 被引量:6

Study on the Role of EPS and LPS of Ralstonia solanacearum in Eucalyptus urophylla×E. grandis Root Adsorption and Ingression
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摘要 通过测定青枯菌的根表吸附量和根部侵入量,研究了胞外多糖(EPS)和脂多糖(LPS)在青枯菌对尾巨桉根部吸附和侵入过程中的作用。结果表明:在接种青枯菌的6 h内,EPS处理后尾巨桉根部青枯菌GN1和93B的吸附量和侵入量明显升高,而LPS处理后尾巨桉根部GN1和93B的吸附量和侵入量都明显下降;无EPS菌株的根表吸附量和根部侵入量都明显降低,无EPS及LPS菌株的根表吸附量和根部侵入量亦有所下降,但没有无EPS菌株下降明显;EPS具有促进青枯菌对尾巨桉根部吸附和侵入的作用,LPS则具有抑制作用。 In this experiment, the role of extracellular polysaccharides (EPS) and lipopolysaccharides (LPS) were studied by detecting the number of Ralstonia solanacearum in Eucalyptus urophylla × E. grandis root adsorption and ingression. The results showed that the number of GN1 and 93B in adsorption and ingression increased obviously when the roots were treated with EPS, while decreased evidently with LPS in 6 h after inoculation. The number of bacteria washed off EPS decreased evidently in adsorption and ingression to the roots. In comparison, the number of bacteria washed off both EPS and LPS had the same decreasing tendency, but not so evident as the former. These results demonstrated that EPS played a role in enhancing the adsorption and ingression of R. solanacearum to the roots of Eucalyptus urophylla × E. grandis, while LPS had inhibitory effect.
出处 《林业科学研究》 CSCD 北大核心 2007年第2期176-180,共5页 Forest Research
基金 国家科技攻关项目"南方主要速生阔叶树种新品种选育及栽培技术(2004BA515B02)" 科技部成果转化项目"南方主要速生阔叶树种新品种及高效培育技术中试" 国家自然科学基金"青枯菌对桉树的识别侵染及其生化机制研究(30471395)"
关键词 青枯菌 胞外多糖 脂多糖 尾巨桉 吸附 侵入 Ralstonia solanacearum EPS LPS Eucalyptus urophylla × E. grandis adsorption ingression
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