摘要
研制了一种利用全血来测试尿酸含量的安培型生物传感器。采用铁氰化钾作为媒介体，将尿酸氧化酶固定在羧甲基纤维素钠处理过的碳电极表面，研制成一种尿酸生物传感器。该传感器在恒电位0．3V和尿酸氧化酶的催化作用下，使被检测物尿酸氧化，铁氰化钾还原，在电极表面产生氧化-还原峰，利用安培法可对尿酸进行间接测定。该传感器经临床测试，尿酸的测定范围可达25～200mg／L，10s内即可达到稳态电流，测试结果的线性范围较好，相关系数为0．9987。另外，尿酸传感器具有好的抗干扰性，测试精度高、重复性好，RSD〈2％。
An amperometric uric acid biosensor was developed by immobilizing uric acid oxidase on carbon electrodes, in which, potassium ferricyanide was used to electron medium of chemistry response. The uric acid measured was oxidized and potassium ferricyanide was deacidized on the biosensor through catalysis of uric acid oxidase and supply of stationary 0.3 volt. As a result, the peak of oxidation-reduction was produced on the biosensor. The concentration of uric acid in product was measured indirectly by amperometry. The range of measuring uric acid was about 25 -200 mg/L from the clinical experiment. It has better linear relationship with correlation coefficient of 0. 9987. It reaches to steady value of currents in 10 seconds. It can be used for the analysis of blood with higher precision, the RSD is less than 2%.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2007年第4期615-618,共4页
Chinese Journal of Analytical Chemistry
基金
中南大学博士后科学基金资助项目(No20060120)
