摘要
用酶联免疫检验技术(ELISA)检测呋喃唑酮(Furazolidone)代谢物AOZ。AOZ经过16h的衍生后和酶联结合物对微孔中的抗体进行竞争反应,利用抗原与抗体的特异性免疫化学反应的基本原理,在酶标仪上450nm处测定吸光强度,进行定性定量分析。
The enzyme-linked immunosorbent assay （ELISA）method for the determination of furazolidone metabolite residues in aquatic products has been studied. After furazolidone has been metabolized for sixteen hours, AOZ and the combination of the enzyme was competed for the antibody in the microaperture. Utilized the fundamental of the specific immunity reaction between antigen and antibody. The absorbance can be determined on 450 nm,then the quantitative analysis was proceed and the nature was determined.
出处
《食品研究与开发》
CAS
北大核心
2007年第5期124-126,共3页
Food Research and Development
基金
长江学者和创新团队发展计划资助(批准号:IRT0540)
关键词
酶联技术
水产品
呋喃唑酮代谢物
AOZ
ELISA
aquatic products
furazolidone
3-amina-2-oxazolldimone（AOZ）
