摘要
本文研究了淘汰奶牛卵巢采集方法、卵丘细胞和颗粒细胞对卵母细胞体外受精后发育的影响。结果表明奶牛屠宰后取其卵巢,用剖解法可比抽吸法得到更多的可用卵母细胞(8．5: 6．2枚／卵巢,P＜0．05)。经成熟培养和体外受精后,无卵丘细胞包围的裸露卵母细胞卵裂率为40．2%,但仅28．2%停留在8～16细胞期,最高发育阶段为16细胞期;而有3层以上卵丘细胞紧密包围的卵丘—卵母细胞复合体,其卵裂率(60．2%:53．2%,P＜0．05)和囊胚发育率(31．5%:19．6%,P＜0．05)均高于卵丘细胞包被不全的卵母细胞。在培养液中添加颗粒细胞,提高囊胚细胞数(96．2±5．2:70．4±4．6,P＜0．05),但没有促进牛受精卵的卵裂率。
This experiment was carried out to study the collect methods of abattoir ovaries, and the effects of cumulus cells and granulose cells on developmental competence of bovine oocytes derived from abattoir ovaries. The result showed that more available oocytes could be obtained in dissected than aspirated method （8. 5/ovary vs 6. 2/ovary, P〈0. 05） after collecting ovaries from slaughtered cows. Following in vitro maturation, fertilization and culture （[VMFC）, the cleavage rate of oocytes without cumulus cells （naked oocytes） from the follicles was 40. 2% with only 28.2% at 8-16 cell stage, the best development was at 16-cell stage; the cleavage rates and blastocyst rates of cumulus and oocytes complex surrounded by more then 3-layer cumulus cells were significantly higher than that of oocytes partly surrounded by cumulus cells （60.2% vs 53. 2% ; 31.5% vs 19. 6%, P〈0. 05）. Addition of granulose cells in the culturing solution could increase the number of blastocytes （96. 2±5. 2：70. 4±4. 6,P〈0. 05）, but had not accelerated the cleavage rate of bovine embryos.
出处
《中国牛业科学》
2007年第2期9-11,共3页
China Cattle Science
基金
广东省自然科学基金项目(编号:05012364)
