摘要
目的:探讨妊娠期糖尿病(GDM)患者骨骼肌组织浆细胞膜糖蛋白-1(PC-1)表达与GDM发病的关系。方法:用Western blot及免疫沉淀法测定GDM20例患者(GDM组)、糖耐量正常孕妇20例(正常妊娠组)及糖耐量正常非孕妇女12例(对照组)骨骼肌组织PC-1、胰岛素受体的表达水平及其基础酪氨酸磷酸化水平和胰岛素刺激后胰岛素受体酪氨酸磷酸化程度。用葡萄糖氧化酶法及放射免疫法测定空腹血葡萄糖(FPG)及空腹血胰岛素(FINS)水平,计算胰岛素抵抗指数(HOMA-IR)。结果:(1)GDM组FPG、FINS、HOMA-IR均明显高于正常妊娠组(P<0.01);正常妊娠组FINS、HOMA-IR明显高于对照组(P<0.01);(2)GDM组PC-1表达水平为1.22±0.02,明显高于正常妊娠组的0.71±0.03及对照组的0.43±0.02(P<0.01);(3)各组胰岛素受体表达水平及基础酪氨酸磷酸化程度比较,差异均无显著性(P>0.05)。胰岛素刺激后酪氨酸磷酸化程度,GDM组为0.17±0.04明显低于正常妊娠组的0.24±0.02(P<0.01),正常妊娠组明显低于对照组的0.31±0.03(P<0.01);(4)正常妊娠组、GDM组PC-1表达水平与HOMA-IR呈明显正相关(r=0.611、0.734,P<0.01),与胰岛素刺激后胰岛素受体酪氨酸磷酸化呈负相关(r=-0.531、-0.522,P<0.05)。结论:骨骼肌组织PC-1高表达可能是妊娠期糖尿病患者胰岛素抵抗的分子机制之一,PC-1可能通过抑制胰岛素受体酪氨酸磷酸化发挥作用。
Objective： To investigate the relationship between plasma cell membrane rotein-1 （PC-1） in skeletal muscle and the etiology of gestational diabetes mellitus. Methods ： The expression of PC-1, insulin receptor and tyrosine phosphorylation in skeletal muscle were determined by Western blot and immunoprecipitation from patients with GDM （GDM group,n = 20 ）, normal pregnant women （ normal pregnancy group, n = 20 ） and nonpregnant women（control group,n = 12）. Fasting plasma glucose （FPG） and fasting insulin （FINS） were measured by oxidized assay and immunoradioassay. Insulin resistant index（HOMA-IR） was calculated with FPG and FINS. Results： （ 1 ） The levels of FPG, FINS, HOMA-IR in GDM group were significantly higher than those in normal pregnancy group （P 〈0.01 ）. The levels of FINS and HOMA-IR in normal pregnancy group were significantly higher than those in control group （P〈0. 01 ）;（2） PC-1 contents in GDM group was higher than those in normal pregnancy group and control group （P 〈 0.01 ） ;（ 3 ）No significant differences were found in insulin receptor content and basal insulin receptor tyrosine phosphorylation in the three groups. After insulin stimulation, insulin receptor tyrosine phosphorylation increased in all subjects but was lower in GDM group compared with normal pregnancy group （P 〈0.01 ） ; （4） PC-1 content was significantly correlated with HOMA-IR （r = 0.734, P 〈 0.01 ） and insulin receptor phosphorylation （r = -0.522 ,P 〈0.05）. Conclusions： PC-1 overexpression in skeletal muscle may be one of the cellular mechanism for insulin resistance of GDM. PC-1 may produce the effect by inhibiting insulin receptor tyrosine phosphorylation.
出处
《现代妇产科进展》
CSCD
北大核心
2007年第3期202-205,共4页
Progress in Obstetrics and Gynecology
关键词
糖尿病
妊娠
胰岛素抗药性
浆细胞膜糖蛋白-1
受体
胰岛素
磷酸化
Diabetes, gestational
Insulin resistance
Plasma cell membrane glyeoprotein-1
Receptor, insulin
Phosphorylation
