摘要
目的:探讨原发性肺癌中几种与多药耐药相关因子的表达及与临床病理的相关性。方法:采用免疫组化方法对术前未经化疗、术后病理诊断明确的133例肺癌组织标本和15例作为对照的癌旁肺组织(距肿瘤边缘5cm),进行了P-gp、MRP、LRP、p53及c-erbB-2蛋白表达的检测。结果:(1)133例肺癌组织中,P-gp、MRP、LRP、p53及c-erbB-2的阳性率分别为72.2%(96/133)、40.5%(51/126)、54.1%(72/126)、59.4%(79/126)和45.9%(61/133),除P-gp外均显著高于正常肺组织中的表达水平(P<0.05);且p53表达与P-gp及MRP表达之间(r=0.179及0.185,P=0.039及0.041),P-gp与LRP之间(r=0.264,P=0.002),LRP与MRP之间(r=0.309,P<0.001),以及c-erbB-2表达分别与MRP、LRP及p53表达之间(r分别为:0.350、0.421、0.541和0.354,P值均<0.001)均有相关性。(2)P-gp、MRP、LRP和c-erbB-2蛋白表达在不同病理类型之间具有统计学差异,而LRP蛋白表达在不同病理分化程度之间具有统计学差异(P<0.001),P-gp在鳞癌不同分化程度间表达有统计学差异(P=0.049)。(3)针对病理类型及亚型:①神经内分泌癌与非神经内分泌癌相比,后者的LRP和c-erbB-2蛋白表达水平较前者高(P=0.005和0.041);②未发现细支气管肺泡癌与其他类型腺癌间在MDR相关因子的蛋白表达水平上有统计学差异。结论:这些多药耐药相关因子可能均在肺癌的原发性多药耐药中起不同程度的作用,且在多药耐药形成以及肿瘤进展的过程中它们之间有某种程度的关联。
Objective：To investigate the expression and clinicopathological significance of several kinds of muitidrug-resistance related factors in primary lung carcinoma. Methods：One hundred and thirty three cases of lung carcinoma tissues confirmed by pathological examination after surgery were included in the study and 15 cases of pericancerous tissues （5-cm apart from tumor） were selected as control. The expressions of P-gp （P-glycoprotein）, multidrug resistance-associated protein （MRP）, lung resistance-related protein （ LRP）, p.53, and c-erbB2 protein in paraffin-embedded tissues were detected by immunohistocbemistry. Results： （ 1 ） The positive rates of P-gp, MRP, LRP, p.53 and c-erbB2 in tumor tissues were 72.2% （96/133）, 40. 5% （51/126）, 54.1% （72/126）, 59.4% （79/126）, and 45.9% （ 61/133 ）, respectively, which were significantly higher than that in normal controls （ P 〈 0.05 ） except for the P-gp （P 〉0.05）. The expression of p.53 correlated with P-gp and MRP （r=0. 179 and0. 185, P =0.039 and 0.041）. There were significant correlation between P-gp and LRP （ r = 0.264, P = 0.002） as well as LRP and MRP （ r = 0. 309, P 〈 0.001 ）. The expres- sion of c-erbB-2 was associated with that of MRP, LRP, and p53 （ r = 0. 350, 0. 421,0. 541, and 0. 354, respectively; P 〈 0.001 ）. （2） There was significant difference among the different pathologic types in P-gp, MRP, LRP, and c-erbB-2 expression （P 〈0. 001 ）. The difference in P-gp expression was significant in lung squamous carcinoma at different differentiation degree （ P = 0.049）. （ 3 ） Ac- cording to pathological classification/sub-classification ： （1）The expression levels of LRP and c-erbB-2 were higher in the panel of neuroendocrine carcinoma than that in the panel of non neuroendocrine carcinoma （P =0. 005 and 0.041, respectively） ; （2）There was no significant difference in the expression of MDR-related factors between the panel of bronchioloalveolar carcinoma and other types of adenocarcinoma. Conclusion： These multidrug-resistance relate factors play important roles in the intrinsic multidrug resistance of lung cancer in different degrees. They have some correlations between each other in the formation of multidurg resistance and the progression of lung cancer.
出处
《肿瘤》
CAS
CSCD
北大核心
2007年第4期303-307,326,共6页
Tumor
